Search for a poster by keyword

Single stage reversed-phase liquid chromatography-mass spectrometry for the characterization of triglyceride positional isomers.
Haslina Hasan, Brendan J Keely and Julie Wilson. University Of York

The high level of selectivity afforded by RP-HPLC in combination with CID allowed recognition of eight groups of TAG positional isomers. Development of UPLC approach has reduced the run time by half while maintaining separation. The improved method combined with principal components analysis was used to assess the extent of variation of major TAGs in subcutaneous and intermuscular pork fat from four different locations of adipose tissues.

An improved data processing pipe-line for comprehensive H-NMR and X/MS -omics data
R. J. O. Torgrip, K. M. Åberg, E. Alm. Dept. Analytical Chemistry

In the post acqusition analysis of comprehensive -omics data the pre-processing pipe-line is crucial to extract the maximum possible amount of information from the data. Here we show a processing pipe-line for (1D)H-NMR and (2D)X/MS data comprising; feature detection, inter-sample feature alignment and internal-standard free normalization that outperforms today’s state-of-the-art processing schemes.

Five noncovalent peptidic ligands show different affinity rankings in solution and gas phase
Andrey Dyachenko , Michael Goldflam , Marta Vilaseca , Ernest Giralt . Institute for Research in Biomedicine (IRB), Barcelona Science Park, University of Barcelona, Spain

Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.

Five noncovalent peptidic ligands show different affinity rankings in solution and gas phase
Andrey Dyachenko , Michael Goldflam , Marta Vilaseca , Ernest Giralt . Institute for Research in Biomedicine (IRB), Barcelona Science Park, University of Barcelona, Spain

Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.

Mass-Spectrometric Analysis with Sequenom EpiTYPER of GNAS Methylation in Pseudohypoparathyroydism Type Ib Patients Reveals Overall Methylation Defects also for the Familial Cases
Benedetta Izzi1, Bart Claes2, Diether Lambrechts2, Chris Van Geet1,3, Kathleen Freson1. 1 Center for Molecular and Vascular Biology, 2 Vesalius Research Center, 3 Department of Pediatrics University Hospital, University of Leuven, Leuven, Belgium

Sequenom EpiTYPER analysis of GNAS methylation in Pseudohypoparathyroidism type Ib patients reveals overall GNAS methylation defects also for the familial cases. Such abnormalities are not detectable via old methodologies such as PCR followed by methylation specific restriction digestion and are here for the first time described.

Force Spectroscopy of the Iron Atom in Heme Proteins
J. T. Sage, B M. Leu, T. H. Ching, Y. Zhang, J. E. Straub, J. Zhao, W. Sturhahn, E. E. Alp. Northeastern University, Boston University, Argonne National Laboratory

Nuclear resonance vibrational spectroscopy (NRVS) selectively reveals the complete vibrational density of states (VDOS) of a Mössbauer probe nucleus within a protein. Frequency moments of the VDOS determine effective force constants for 57Fe at the active sites of cytochrome c (cyt c) and deoxymyoglobin (Mb).

 1 - 6 of 41 Next