The noncanonical heme oxygenase MhuD from Mycobacterium tuberculosis binds a heme substrate that adopts a dynamic equilibrium between planar and out-of-plane ruffled conformations. MhuD degrades this substrate to an uncommon mycobilin product through successive monooxygenation and dioxygenation reactions. This article establishes a causal relationship between heme substrate dynamics and MhuD-catalyzed heme degradation, leading to a refined enzymatic mechanism. UV/vis absorption (Abs) and electrospray ionization mass spectrometry (ESI-MS) information demonstrated {that a} second-sphere substitution favoring the inhabitants of the ruffled heme conformation modified the rate-limiting step of the response, leading to a measurable buildup of the monooxygenated meso-hydroxyheme intermediate.
In addition, UV/vis Abs and ESI-MS information for a second-sphere variant that favored the planar substrate conformation confirmed that this modification altered the enzymatic mechanism leading to an α-biliverdin product. Single-turnover kinetic analyses for three MhuD variants revealed that the speed of heme monooxygenation relies upon upon the inhabitants of the ruffled substrate conformation. These kinetic analyses additionally revealed that the speed of meso-hydroxyheme dioxygenation by MhuD relies upon upon the inhabitants of the planar substrate conformation. Thus, the ruffled heme conformation helps speedy heme monooxygenation by MhuD, however additional oxygenation to the mycobilin product is inhibited. In distinction, the planar substrate conformation displays altered heme monooxygenation regiospecificity adopted by speedy oxygenation of meso-hydroxyheme.
Library looking is a robust method for detecting peptides utilizing both information impartial or information dependent acquisition. While each large-scale spectrum library curators and deep studying prediction approaches have centered on beam-type CID fragmentation (HCD), resonance CID fragmentation stays a preferred method. Here we reveal an strategy to mannequin the variations between HCD and CID spectra, and current a software program instrument, CIDer, for changing libraries between the 2 fragmentation strategies. We reveal that simply utilizing a mix of easy linear fashions and fundamental ideas of peptide fragmentation, we are able to clarify up to 43% of the variation between ions fragmented by HCD and CID throughout an array of collision vitality settings.
We additional present that in some circumstances, looking transformed CID libraries can detect extra peptides than looking current CID libraries or libraries of machine studying predictions from FASTA databases. These outcomes recommend that leveraging data in current libraries by changing between HCD and CID libraries could also be an efficient interim resolution whereas large-scale CID libraries are being developed. Altogether, these information yielded a refined enzymatic mechanism for MhuD the place entry to each substrate conformations is wanted for speedy incorporation of three oxygen atoms into heme yielding mycobilin. Understanding how these multi-layered molecular networks modulate insulin motion and metabolism in numerous tissues will open new avenues for remedy and prevention of kind 2 diabetes and its related pathologies.
Proteomics in getting old analysis: A roadmap to medical, translational analysis
The identification of plasma proteins that systematically change with age and, impartial of chronological age, predict accelerated decline of well being is an increasing space of analysis. Circulating proteins are superb translational “omics” since they’re last effectors of physiological pathways and since physicians are accustomed to use data of plasma proteins as biomarkers for analysis, prognosis, and monitoring the effectiveness of remedies. In this regard, research utilizing human induced pluripotent stem cells and tissues level to cell-autonomous alterations in signalling super-networks, involving adjustments in phosphorylation and gene expression each inside and out of doors the canonical insulin signalling pathway.
Recent technological developments, together with mass spectrometry (MS)-based proteomics, multiplexed proteomic assay utilizing modified aptamers (SOMAscan), and Proximity Extension Assay (PEA, O-Link), have allowed for the evaluation of 1000’s of proteins in plasma or different organic matrices, that are doubtlessly translatable into new medical biomarkers and supply new clues in regards to the mechanisms by which getting old is related to well being deterioration and purposeful decline. We carried out an in depth literature search for proteomic research carried out in numerous matrices (plasma, serum, urine, saliva, tissues) and species utilizing a number of platforms.
Herein, we recognized 232 proteins that have been age-associated throughout research. Enrichment evaluation of the 232 age-associated proteins revealed metabolic pathways beforehand related with organic getting old each in animal fashions and in people, most remarkably insulin-like progress issue (IGF) signaling, mitogen-activated protein kinases (MAPK), hypoxia-inducible issue 1 (HIF1), cytokine signaling, Forkhead Box O (FOXO) metabolic pathways, folate metabolism, advance glycation finish merchandise (AGE), and receptor AGE (RAGE) metabolic pathway. Information on these age-relevant proteins, seemingly expanded and validated in longitudinal research and examined in mechanistic research, shall be important for affected person stratification and the event of new remedies aimed toward bettering well being expectancy.
Defining the underlying defect in insulin motion in kind 2 diabetes
Insulin resistance is one of the earliest defects within the pathogenesis of kind 2 diabetes. Over the previous 50 years, elucidation of the insulin signalling community has offered necessary mechanistic insights into the abnormalities of glucose, lipid and protein metabolism that underlie insulin resistance. In classical goal tissues (liver, muscle and adipose tissue), insulin binding to its receptor initiates a broad signalling cascade mediated by adjustments in phosphorylation, gene expression and vesicular trafficking that end in elevated nutrient utilisation and storage, and suppression of catabolic processes.
Fetal Uterus Lysate |
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| XBL-10431 | ProSci | 0.1 mg | EUR 527 |
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Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Uterus Membrane Lysate |
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| XBL-11023 | ProSci | 0.1 mg | EUR 516.5 |
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Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Uterus Tumor Lysate |
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| 1384 | ProSci | 0.1 mg | EUR 254 |
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Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Rabbit SCLERA YG FROZEN 1 EA |
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| 41216-1 | Pel-Freez | 1 Each | EUR 83.49 |
Rabbit HEART YG FROZEN 50 EA |
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| 41218-2 | Pel-Freez | 50 Each | EUR 342.31 |
Rabbit MUSCLE TISSUE MT FROZEN 1kg |
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| 41325-2 | Pel-Freez | 1 kg | EUR 281.41 |
Bovine UTERUS 5 EA* |
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| 57153-2 | Pel-Freez | 5 EA | EUR 204.2 |
Human Uterus Tumor lysate |
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| HTL-1384 | Alpha Diagnostics | 1 mg | EUR 773 |
Uterus Tissue Slide (Normal) |
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| 11-401-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Normal) |
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| 11-401-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Benign) |
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| 11-402-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Benign) |
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| 11-402-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Adenocarcinoma) |
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| 11-404-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Adenocarcinoma) |
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| 11-404-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Abnormal) |
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| 11-419-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Abnormal) |
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| 11-419-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Adenomyoma) |
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| 11-432-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Adenomyoma) |
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| 11-432-4um | ProSci | 4 um | EUR 180.5 |
Uterus Membrane Tumor Lysate |
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| XBL-11031 | ProSci | 0.1 mg | EUR 626.75 |
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Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Uterus-Corpus Membrane Lysate |
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| XBL-11037 | ProSci | 0.1 mg | EUR 516.5 |
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Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Uterus-Fundus Membrane Lysate |
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| XBL-11040 | ProSci | 0.1 mg | EUR 516.5 |
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Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Control Sections for FD NeuroSilver™ Kit |
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| PCS101 | FD Neurotechnologies | each | EUR 58 |
Fine natural hair brush for mounting sections |
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| PO501 | FD Neurotechnologies | each | EUR 67 |
CancerSeq? Plus Paraffin Tissue Tumor Sections: Breast |
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| T2235086-SC | Biochain | 5 curls | EUR 932 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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CancerSeq? Plus Paraffin Tissue Tumor Sections: Stomach |
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| T2235248-SC | Biochain | 5 curls | EUR 932 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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CancerSeq? Plus Paraffin Tissue Tumor Sections: Thyroid |
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| T2235265-SC | Biochain | 5 curls | EUR 932 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus |
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| R1234275-50 | Biochain | 50 ug | EUR 178 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus |
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| R1234276-10 | Biochain | 10 ug | EUR 194 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Rabbit PSOAS MUSCLE YG FROZEN 1 EA |
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| 41217-1 | Pel-Freez | 1 Each | EUR 180.28 |
Total RNA from Lupus: Uterus |
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| R1236274Lup-50 | Biochain | 50 ug | EUR 351 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Uterus Tissue Slide (Endometrioid Adenocarcinoma) |
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| 11-405-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Endometrioid Adenocarcinoma) |
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| 11-405-4um | ProSci | 4 um | EUR 180.5 |
Control sections for FD NeuroApop™ Kit (paraffin) |
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| PCS102-1 | FD Neurotechnologies | each | EUR 67.03 |
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Description: Best quality products |
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FD NeuroSilver™ Kit II (for 300 sections) |
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| PK301 | FD Neurotechnologies | kit | EUR 792 |
FD NeuroSilver™ Kit II (for 150 sections) |
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| PK301A | FD Neurotechnologies | kit | EUR 658 |
Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus |
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| R1234275-P | Biochain | 50 ug | EUR 328 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Tumor Tissue: Uterus |
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| C1235274 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Uterus Tissue Slide ( Papillary serous Adenocarcinoma) |
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| 11-407-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide ( Papillary serous Adenocarcinoma) |
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| 11-407-4um | ProSci | 4 um | EUR 180.5 |
Uterus Tissue Slide (Spindle Cell Tumor) |
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| 11-418-10um | ProSci | 10 um | EUR 201.5 |
Uterus Tissue Slide (Spindle Cell Tumor) |
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| 11-418-4um | ProSci | 4 um | EUR 180.5 |
Monkey (Rhesus) Normal Uterus Whole tissue lysate |
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| MRL-1332 | Alpha Diagnostics | 1 mg | EUR 773 |
Monkey (Cyno.) Normal Uterus Whole tissue lysate |
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| MCL-1222 | Alpha Diagnostics | 1 mg | EUR 773 |
Human Adult Uterus (Normal) Whole tissue lysate |
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| HAL-1317 | Alpha Diagnostics | 1 mg | EUR 524 |
cDNA from Human Adult Normal Tissue: Uterus |
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| C1234274 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Uterus |
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| C1534274 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus |
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| C1534274-Cy | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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FFPE Genomic DNA - Human Tumor Tissue: Uterus |
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| D2235274 | Biochain | 2 ug | EUR 821 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Total Protein from Human Tumor Tissue: Uterus |
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| P1235274 | Biochain | 1 mg | EUR 320 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Membrane Protein from Human Tumor Tissue: Uterus |
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| P3235274 | Biochain | 0.1 mg | EUR 408 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Total RNA from Human Tumor Tissue: Uterus |
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| R1235274-50 | Biochain | 50 ug | EUR 351 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Paraffin Tissue Section - Human Adult Normal: Uterus |
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| T2234274 | Biochain | 5 slides | EUR 211 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section - Human Uterus Cervix Tumor |
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| T2235275 | Biochain | 5 slides | EUR 257 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Lupus: Adrenal |
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| T1236004Lup | Biochain | 5 slides | EUR 474 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Arteriosclerosis: Aorta |
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| T1236012Hd-4 | Biochain | 5 slides | EUR 976 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - hypertension: Artery |
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| T1236013Hd-2 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Arteriosclerosis: Artery |
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| T1236013Hd-4 | Biochain | 5 slides | EUR 976 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - hypertension: Vein |
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| T1236020Hd-2 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - hypertension: Heart |
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| T1236122Hd-2 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - hypertension: Kidney |
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| T1236142Hd-2 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Lupus: Kidney |
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| T1236142Lup | Biochain | 5 slides | EUR 534 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Asthma: Lung |
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| T1236152Ld-1 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Bronchitis: Lung |
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| T1236152Ld-2 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Emphysema: Lung |
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| T1236152Ld-3 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Pneumonia: Lung |
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| T1236152Ld-4 | Biochain | 5 slides | EUR 465 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Lupus: Ovary |
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| T1236183Lup | Biochain | 5 slides | EUR 611 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Lupus: Pancreas |
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| T1236188Lup | Biochain | 5 slides | EUR 474 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Lupus: Spleen |
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| T1236246Lup | Biochain | 5 slides | EUR 474 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Array - Rabbit Male Adult Normal Tissue, Multi-tissue I |
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| T6834700-G01-1 | Biochain | 1 slide | EUR 258 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Array - Rabbit Male Adult Normal Tissue, Multi-tissue I |
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| T6834700-G01-5 | Biochain | 5 slides | EUR 821 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I |
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| T6834700-G02-1 | Biochain | 1 slide | EUR 258 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Array - Rabbit Female Adult Normal Tissue, Multi-tissue I |
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| T6834700-G02-5 | Biochain | 5 slides | EUR 821 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Human Uterus Tissue Slide (Normal) (5 slides/pk) |
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| HTS-11401 | Alpha Diagnostics | 1 pk | EUR 286 |
Human Uterus Tissue Slide (Benign) (5 slides/pk) |
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| HTS-11402 | Alpha Diagnostics | 1 pk | EUR 286 |
Human Uterus Tissue Slide (Adenocarcinoma) (5 slides/pk) |
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| HTS-11404 | Alpha Diagnostics | 1 pk | EUR 286 |
Human Uterus Tissue Slide (Abnormal) (5 slides/pk) |
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| HTS-11419 | Alpha Diagnostics | 1 pk | EUR 286 |
Human Uterus Tissue Slide (Adenomyoma) (5 slides/pk) |
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| HTS-11432 | Alpha Diagnostics | 1 pk | EUR 286 |
cDNA from Human Adult Normal Tissue: Uterus: Cervix |
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| C1234275 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Uterus: Corpus |
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| C1234276-10 | Biochain | 10 reactions | EUR 231 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Human Adult Normal Tissue: Uterus: Fundus |
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| C1234278-10 | Biochain | 10 reactions | EUR 231 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Cervix |
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| C1534275 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix |
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| C1534275-Cy | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus |
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| C1534276 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus |
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| C1534278 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Total Protein from Human Adult Normal Tissue: Uterus |
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| P1234274 | Biochain | 1 mg | EUR 214 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Membrane Protein from Human Adult Normal Tissue: Uterus |
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| P3234274 | Biochain | 0.1 mg | EUR 285 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Total RNA from Human Adult Normal Tissue: Uterus |
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| R1234274-50 | Biochain | 50 ug | EUR 178 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Paraffin Tissue Section - Human Uterus Tumor: Endometrium Adenocarcinoma |
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| T2235274-3 | Biochain | 5 slides | EUR 257 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Matching Pair - Paraffin Tissue Sections - Human Primary Tumor and Normal (PP): Stomach |
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| T8235248-PP | Biochain | 5 slides x2 | EUR 403 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Zenoquick Frozen-EZ Yeast Transformation Kit |
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| Z6002-001 | GenDepot | 1Kit | EUR 226 |
Frozen Tissue Section - Human Tumor: Adrenal |
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| T1235004 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Tumor: Bladder |
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| T1235010 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Tumor: Bone |
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| T1235023 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Tumor: Brain |
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| T1235035 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Insulin receptors are additionally expressed in non-classical targets, such because the mind and endothelial cells, the place it helps regulate urge for food, vitality expenditure, reproductive hormones, temper/behaviour and vascular operate. Recent progress in cell biology and unbiased molecular profiling by mass spectrometry and DNA/RNA-sequencing has offered a novel alternative to dissect the determinants of insulin resistance in kind 2 diabetes and the metabolic syndrome; finest studied are extrinsic elements, comparable to circulating lipids, amino acids and different metabolites and exosomal microRNAs. More difficult has been defining the cell-intrinsic elements programmed by genetics and epigenetics that underlie insulin resistance.