Author: Barry Alexander

The antibodies are often times a determining factor for success and reproducibility of the tests. Today’s large commercial supply of antibodies makes it not always easy to choose the one that works well in a specific application . Many of you frequently ask us about the key aspects to look for in selecting the most suitable antibody . So today we bring you this short … Read more

Proteins need specific conditions to maintain their conformational, stability and activity characteristics . Unfavorable conditions or sudden changes in the protein’s native environment can lead to the protein being degraded, denatured or precipitated. In this post we will review the factors that influence the stability of proteins, as well as some guidelines to know how to store proteins optimally, thus … Read more

The production of polyclonal antibodies is carried out from a mixture of B lymphocytes with an affinity for different epitopes of the same antigen. And it is precisely this characteristic, the ability to recognize multiple epitopes of a certain antigen , which is an advantage over monoclonal antibodies in some assays, such as immunoprecipitation. In this post, we will review … Read more

Matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF MS)-based direct-on-target microdroplet progress assay (DOT-MGA) was not too long ago described as a novel methodology of phenotypic antimicrobial susceptibility testing (AST). Here, we developed the appliance of MALDI-TOF MS-based DOT-MGA for Gram-positive micro organism together with AST from agar cultures and instantly from optimistic blood cultures (BCs) utilizing … Read more