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Isomerization of trans-3-methylglutaconic acid

Isomerization of trans-3-methylglutaconic acid

3-Methylglutaconic (3MGC) aciduria is a typical phenotypic function of a rising quantity of inborn errors of metabolism. “Primary” 3MGC aciduria is attributable to deficiencies in leucine pathway enzymes whereas “secondary” 3MGC aciduria outcomes from inborn errors of metabolism that impression mitochondrial vitality manufacturing. The metabolic precursor of 3MGC acid is trans-3MGC CoA, an intermediate within the leucine catabolism pathway. Gas chromatography-mass spectrometry (GC-MS) evaluation of commercially out there trans-3MGC acid yielded a combination of cis and trans isomers whereas 1H-NMR spectroscopy of trans-3MGC acid at 25°C supplied no proof for the cis isomer.

When trans-3MGC acid was incubated beneath circumstances used for pattern derivatization previous to GC-MS (however with no trimethylsilane added), 1H-NMR spectroscopy supplied proof of trans to cis isomerization. Incubation of trans-3MGC acid at 37°C resulted in time-dependent isomerization to cis-3MGC acid. Cis-3MGC acid behaved in the same method besides that, beneath equivalent incubation circumstances, much less isomerization occurred. In settlement with these experimental outcomes, molecular modeling research supplied proof that the vitality minimized construction of cis-3MGC acid is four kJ/mol extra secure than that for trans-3MGC acid.

The knowledge introduced are according to the incidence of each diastereomers in urine samples of topics with 3MGC aciduria. Quantification of iron is a crucial step to evaluate the iron burden in sufferers affected by iron overload illnesses, in addition to super worth in understanding the underlying function of iron within the pathophysiology of these illnesses. Current iron willpower of complete or labile iron, requires in depth pattern dealing with and specialised devices, while being time consuming and laborious. Moreover, there’s minimal to no overlap between complete iron and labile iron quantification methodologies-i.e. requiring totally separate protocols, strategies and devices. Herein, we report a unified-ferene (u-ferene) assay that allows a 2-in-1 quantification of each labile and complete iron from the identical preparation of a organic specimen.

MANTI: Automated Annotation of Protein N-Termini for Rapid Interpretation of N-Terminome Data Sets

Site-specific proteolytic processing is a crucial, irreversible post-translational protein modification with implications in lots of illnesses. Enrichment of protein N-terminal peptides adopted by mass spectrometry-based identification and quantification allows proteome-wide characterization of proteolytic processes and protease substrates however is challenged by the shortage of particular annotation instruments. A typical downside is, for instance, ambiguous matches of recognized peptides to a number of protein entries within the databases used for identification.

We developed MaxQuant Advanced N-termini Interpreter (MANTI), a standalone Perl software program with an optionally available graphical consumer interface that validates and annotates N-terminal peptides recognized by database searches with the favored MaxQuant software program bundle by integrating data from a number of knowledge sources. MANTI makes use of numerous annotation data in a multistep determination course of to assign a conservative most well-liked protein entry for every N-terminal peptide, enabling automated classification in line with the doubtless origin and determines important adjustments in N-terminal peptide abundance. Auxiliary R scripts included within the software program bundle summarize and visualize key facets of the information.

To showcase the utility of MANTI, we generated two large-scale TAILS N-terminome knowledge units from two completely different animal fashions of chemically and genetically induced kidney illness, puromycin adenonucleoside-treated rats (PAN), and heterozygous Wilms Tumor protein 1 mice (WT1). MANTI enabled fast validation and autonomous annotation of >10 000 recognized terminal peptides, revealing novel proteolytic proteoforms in 905 and 644 proteins, respectively. Quantitative evaluation indicated that proteolytic actions with related sequence specificity are concerned within the pathogenesis of kidney harm and proteinuria in each fashions, whereas coagulation processes and complement activation have been particularly induced after chemical harm.

Isomerization of trans-3-methylglutaconic acid

ALS/FTD-causing mutation in cyclin F causes the dysregulation of SFPQ

Previously, we recognized missense mutations in CCNF which can be causative of familial and sporadic amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Hallmark options of these illnesses embody the build-up of insoluble protein aggregates in addition to the mislocalisation of proteins reminiscent of TDP-43. In latest years, the dysregulation of SFPQ has additionally emerged as a pathological hallmark of ALS/FTD. CCNF encodes for the protein cyclin F, a substrate recognition part of an E3-ubiquitin ligase. We have beforehand proven that ALS/FTD-linked mutations in CCNF trigger disruptions to general protein homeostasis that results in a build-up of Ok48-linked ubiquitylated proteins in addition to defects in autophagic equipment.

To examine additional processes that could be affected by cyclin F, we used a protein-proximity ligation methodology, often known as BioID, customary immunoprecipitations and mass spectrometry (MS) to establish novel interplay companions of cyclin F and infer additional course of that could be affected by the ALS/FTD-causing mutation. Results display that cyclin F intently associates with proteins concerned with RNA metabolism in addition to a quantity of RNA binding proteins beforehand linked to ALS/FTD, together with splicing issue proline and glutamine wealthy (SFPQ).

RPA Interacting Protein Antibody

4015-01mg 0.1 mg
EUR 436.42
Description: RPA Interacting Protein Antibody: Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.

RPA Interacting Protein

20-abx262685
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

RPA-Interacting Protein (RPAIN) Antibody

abx028958-400ul 400 ul
EUR 523

RPA-Interacting Protein (RPAIN) Antibody

abx028958-80l 80 µl
EUR 286

RPA-Interacting Protein (RPAIN) Antibody

abx237394-100ug 100 ug
EUR 551

RPA Interacting Protein Peptide

4013P 0.05 mg
EUR 164.75
Description: (CT) RPA Interacting Protein peptide

RPA Interacting Protein Peptide

4015P 0.05 mg
EUR 164.75
Description: (NT) RPA Interacting Protein peptide

RPAIN RPA Interacting Protein Human Recombinant Protein

PROTQ86UA6 Regular: 10ug
EUR 317
Description: RPAIN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 129 amino acids (1-106 a.a.) and having a molecular mass of 14.7kDa.RPAIN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Human RPA-interacting protein (RPAIN) ELISA Kit

abx382885-96tests 96 tests
EUR 911

Mouse RPA-interacting protein (RPAIN) ELISA Kit

abx390479-96tests 96 tests
EUR 911

Rat RPA-interacting protein (RPAIN) ELISA Kit

abx391921-96tests 96 tests
EUR 911

Mouse RPA- interacting protein, Rpain ELISA KIT

ELI-42588m 96 Tests
EUR 865

Human RPA- interacting protein, RPAIN ELISA KIT

ELI-22025h 96 Tests
EUR 824

Rpain ELISA Kit| Mouse RPA-interacting protein ELISA Kit

EF016121 96 Tests
EUR 689

Rpain ELISA Kit| Rat RPA-interacting protein ELISA Kit

EF019281 96 Tests
EUR 689

RPA p32 (phospho Ser33) Polyclonal Antibody

ES1439-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against RPA p32 (phospho Ser33) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

RPA p32 (phospho Ser33) Polyclonal Antibody

ES1439-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against RPA p32 (phospho Ser33) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

RPA p32 (phospho Ser33) Polyclonal Antibody

ABP50440-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of RPA p32 phospho Ser33) from Human, Mouse, Rat. This RPA p32 phospho Ser33) antibody is for WB , ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human RPA p32 around the phosphorylation site of S33

RPA p32 (phospho Ser33) Polyclonal Antibody

ABP50440-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of RPA p32 phospho Ser33) from Human, Mouse, Rat. This RPA p32 phospho Ser33) antibody is for WB , ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human RPA p32 around the phosphorylation site of S33

RPA p32 (phospho Ser33) Polyclonal Antibody

ABP50440-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of RPA p32 phospho Ser33) from Human, Mouse, Rat. This RPA p32 phospho Ser33) antibody is for WB , ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human RPA p32 around the phosphorylation site of S33

RPA 70 kDa subunit antibody

22833-100ul 100ul
EUR 390

RPA 14 kDa subunit antibody

22947-100ul 100ul
EUR 390

RPA 32 kDa subunit antibody

23069-100ul 100ul
EUR 390

Anti-Phospho-RPA p32 (S33) Antibody

P02067 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Phospho-RPA p32 (S33) Antibody (RPA2) detection. Tested with WB in Human, Mouse, Rat.

Anti-Phospho-RPA p32 (S33) antibody

STJ90636 200 µl
EUR 197
Description: Rabbit polyclonal to Phospho-RPA p32 (S33).

Polyclonal HSC70 Interacting Protein HIP (ST13) Antibody

APR16725G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HSC70 Interacting Protein HIP (ST13) . This antibody is tested and proven to work in the following applications:

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human)

4-PAC803Hu01
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP)

PAK-interacting Protein 1 (PAK1IP1) polyclonal antibody

ABP-PAB-10259 100 ug Ask for price

Huntingtin-associated protein interacting protein (HAPIP) polyclonal antibody

ABP-PAB-10621 100 ug Ask for price

Rabbit Cytohesin Interacting Protein ELISA kit

E04C0575-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Cytohesin Interacting Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytohesin Interacting Protein ELISA kit

E04C0575-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Cytohesin Interacting Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytohesin Interacting Protein ELISA kit

E04C0575-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Cytohesin Interacting Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Hedgehog Interacting Protein ELISA kit

E04H0058-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Hedgehog Interacting Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Hedgehog Interacting Protein ELISA kit

E04H0058-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Hedgehog Interacting Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Hedgehog Interacting Protein ELISA kit

E04H0058-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Hedgehog Interacting Protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Pancreatic Amylase Antibody (Clone RPA-B5)

3102-100
EUR 338

CD4 FITC Monoclonal Antibody (Clone RPA-T4)

6953-100
EUR 251

CD4 FITC Monoclonal Antibody (Clone RPA-T4)

6953-25
EUR 131

Anti-CD2 Antibody [RPA-2.10], APC-100Tests

QAB1-APC-100Tests 100Tests
EUR 369

Anti-CD2 Antibody [RPA-2.10], APC-25Tests

QAB1-APC-25Tests 25Tests
EUR 200

Anti-CD2 Antibody [RPA-2.10], APC-500Tests

QAB1-APC-500Tests 500Tests
EUR 1367

Anti-CD2 Antibody [RPA-2.10], FITC-100Tests

QAB1-F-100Tests 100Tests
EUR 259

Anti-CD2 Antibody [RPA-2.10], FITC-25Tests

QAB1-F-25Tests 25Tests
EUR 157

Anti-CD2 Antibody [RPA-2.10], FITC-500Tests

QAB1-F-500Tests 500Tests
EUR 953

Anti-CD2 Antibody [RPA-2.10], PerCP-100Tests

QAB1-PCP-100Tests 100Tests
EUR 377

Anti-CD2 Antibody [RPA-2.10], PerCP-25Tests

QAB1-PCP-25Tests 25Tests
EUR 182

Anti-CD2 Antibody [RPA-2.10], PerCP-500Tests

QAB1-PCP-500Tests 500Tests
EUR 1444

Anti-CD2 Antibody [RPA-2.10], PE-100Tests

QAB1-PE-100Tests 100Tests
EUR 318

Anti-CD2 Antibody [RPA-2.10], PE-25Tests

QAB1-PE-25Tests 25Tests
EUR 182

Anti-CD2 Antibody [RPA-2.10], PE-500Tests

QAB1-PE-500Tests 500Tests
EUR 1182

Anti-CD2 Antibody [RPA-2.10], V450-100Tests

QAB1-V450-100Tests 100Tests
EUR 411

Anti-CD2 Antibody [RPA-2.10], V450-25Tests

QAB1-V450-25Tests 25Tests
EUR 200

Anti-CD2 Antibody [RPA-2.10], V450-500Tests

QAB1-V450-500Tests 500Tests
EUR 1596

Anti-CD4 Antibody [RPA-T4], Unconjugated-100ug

QAB12-100ug 100ug
EUR 123

Anti-CD4 Antibody [RPA-T4], APC-100Tests

QAB12-APC-100Tests 100Tests
EUR 182

Anti-CD4 Antibody [RPA-T4], APC-25Tests

QAB12-APC-25Tests 25Tests
EUR 123

Anti-CD4 Antibody [RPA-T4], APC-500Tests

QAB12-APC-500Tests 500Tests
EUR 487

Anti-CD4 Antibody [RPA-T4], FITC-100Tests

QAB12-F-100Tests 100Tests
EUR 141

Anti-CD4 Antibody [RPA-T4], FITC-25Tests

QAB12-F-25Tests 25Tests
EUR 115

Anti-CD4 Antibody [RPA-T4], FITC-500Tests

QAB12-F-500Tests 500Tests
EUR 403

Anti-CD4 Antibody [RPA-T4], PE-100Tests

QAB12-PE-100Tests 100Tests
EUR 166

Anti-CD4 Antibody [RPA-T4], PE-25Tests

QAB12-PE-25Tests 25Tests
EUR 115

Anti-CD4 Antibody [RPA-T4], PE-500Tests

QAB12-PE-500Tests 500Tests
EUR 487

Anti-CD4 Antibody [RPA-T4], V450-100Tests

QAB12-V450-100Tests 100Tests
EUR 242

Anti-CD4 Antibody [RPA-T4], V450-25Tests

QAB12-V450-25Tests 25Tests
EUR 141

Anti-CD8a Antibody [RPA-T8], Unconjugated-100ug

QAB19-100ug 100ug
EUR 149

Anti-CD8a Antibody [RPA-T8], APC-100Tests

QAB19-APC-100Tests 100Tests
EUR 182

Anti-CD8a Antibody [RPA-T8], APC-25Tests

QAB19-APC-25Tests 25Tests
EUR 123

Anti-CD8a Antibody [RPA-T8], APC-500Tests

QAB19-APC-500Tests 500Tests
EUR 487

Anti-CD8a Antibody [RPA-T8], FITC-100Tests

QAB19-F-100Tests 100Tests
EUR 141

Anti-CD8a Antibody [RPA-T8], FITC-25Tests

QAB19-F-25Tests 25Tests
EUR 115

Anti-CD8a Antibody [RPA-T8], FITC-500Tests

QAB19-F-500Tests 500Tests
EUR 403

Anti-CD8a Antibody [RPA-T8], PE-100Tests

QAB19-PE-100Tests 100Tests
EUR 182

Anti-CD8a Antibody [RPA-T8], PE-25Tests

QAB19-PE-25Tests 25Tests
EUR 123

Anti-CD8a Antibody [RPA-T8], PE-500Tests

QAB19-PE-500Tests 500Tests
EUR 487

Anti-CD8a Antibody [RPA-T8], V450-100Tests

QAB19-V450-100Tests 100Tests
EUR 233

Anti-CD8a Antibody [RPA-T8], V450-25Tests

QAB19-V450-25Tests 25Tests
EUR 149

Polyclonal Goat Anti-GCIP-interacting protein p29 Antibody

APG00146G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-GCIP-interacting protein p29 . This antibody is tested and proven to work in the following applications:

Huntingtin Interacting Protein 2 (HIP2) Polyclonal Antibody (Human)

4-PAE601Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Huntingtin Interacting Protein 2 (HIP2)

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human), APC

4-PAC803Hu01-APC
  • EUR 333.00
  • EUR 3113.00
  • EUR 872.00
  • EUR 423.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP). This antibody is labeled with APC.

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human), Biotinylated

4-PAC803Hu01-Biotin
  • EUR 303.00
  • EUR 2341.00
  • EUR 697.00
  • EUR 369.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP). This antibody is labeled with Biotin.

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human), Cy3

4-PAC803Hu01-Cy3
  • EUR 403.00
  • EUR 4109.00
  • EUR 1121.00
  • EUR 523.00
  • EUR 245.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP). This antibody is labeled with Cy3.

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human), FITC

4-PAC803Hu01-FITC
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP). This antibody is labeled with FITC.

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human), HRP

4-PAC803Hu01-HRP
  • EUR 305.00
  • EUR 2714.00
  • EUR 772.00
  • EUR 383.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP). This antibody is labeled with HRP.

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human), PE

4-PAC803Hu01-PE
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Toll Interacting Protein (TOLLIP). This antibody is labeled with PE.

Toll Interacting Protein (TOLLIP) Polyclonal Antibody (Human, Mouse)

4-PAC803Mu01
  • EUR 243.00
  • EUR 2457.00
  • EUR 613.00
  • EUR 305.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Toll Interacting Protein (TOLLIP)

Thyroid receptor interacting protein 15 (TRIP15) polyclonal antibody

ABP-PAB-10139 100 ug Ask for price

Fms interacting protein (FMIP) [domain 1] polyclonal antibody

ABP-PAB-11638 100 ug Ask for price

Fms interacting protein (FMIP) [domain 2] polyclonal antibody

ABP-PAB-11639 100 ug Ask for price

Traf Interacting Protein Antibody

20-abx116139
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

HSP70 Interacting Protein Antibody

20-abx137349
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Toll-interacting protein Antibody

20-abx137443
  • EUR 704.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 20 ug
  • 5 ug

Notably, the overexpression of cyclin F(S621G) led to the aggregation and altered subcellular distribution of SFPQ in HEK293 cells, while resulting in altered degradation in major neurons. Overall, our knowledge hyperlinks ALS/FTD-causing mutations in CCNF to converging pathological options of ALS/FTD and supplies a hyperlink between faulty protein degradation programs and the pathological accumulation of a protein concerned in RNA processing and metabolism. Once generated in vivo, trans-3MGC acid is proposed to isomerize through a mechanism involving π electron delocalization with formation of a resonance construction that allows bond rotation.