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Design of Abiological Digital Poly(phosphodiester)s

Design of Abiological Digital Poly(phosphodiester)s

ConspectusIn organic techniques, the storage and switch of genetic data depend on sequence-controlled nucleic acids reminiscent of DNA and RNA. It has been realized for fairly a while that this property isn’t solely essential for all times however is also very helpful in human purposes. For occasion, DNA has been actively investigated as a digital storage medium over the previous decade. Indeed, the “hard-disk of life” is an apparent alternative and a extremely optimized materials for storing knowledge. Through a long time of nucleic acids analysis, technological instruments for parallel synthesis and sequencing of DNA have been available.

Consequently, it has already been demonstrated that differing types of paperwork (e.g., texts, photographs, movies, and industrial knowledge) might be saved in chemically synthesized DNA libraries. However, DNA is topic to organic constraints, and its molecular construction can’t be simply diverse to match technological wants. In reality, DNA isn’t the one macromolecule that permits knowledge storage. In current years, it has been demonstrated that all kinds of artificial polymers will also be used for such a function. Indeed, fashionable polymer synthesis permits the preparation of artificial macromolecules with exactly managed monomer sequences. Altogether, a couple of dozens of artificial digital polymers have already been described, and lots of extra might be foreseen.

Among them, sequence-defined poly(phosphodiester)s are one of probably the most promising choices. These polymers are ready by stepwise phosphoramidite chemistry like chemically synthesized oligonucleotides. However, they’re constructed with non-natural constructing blocks and subsequently share nearly no structural traits with nucleic acids, besides phosphate repeat items. Still, they include readable digital messages that may be deciphered by nanopore sequencing or mass spectrometry sequencing. In this Account, we describe our current analysis efforts in synthesizing and sequencing optimum abiological digital poly(phosphodiester)s. A significant benefit of these polymers over DNA is that their molecular construction can simply be diverse to tune their properties.

During the final 5 years, we have now engineered the molecular construction of these polymers to regulate essential parameters such because the storage density, storage capability, erasability, and readability. Consequently, high-capacity PPDE chains, containing tons of of bits per chains, can now be synthesized and effectively sequenced utilizing a routine mass spectrometer. Furthermore, sequencing knowledge might be routinely decrypted with the assistance of decoding software program. This new sort of coded matter will also be edited utilizing sensible bodily triggers reminiscent of mild and arranged in area by programmed self-assembly. All of these current enhancements are summarized and mentioned herein.

Identification of Protein Direct Interactome with Genetic Code Expansion and Search Engine OpenUaa

Protein crosslinks happen endogenously reminiscent of modifications by ubiquitin-like proteins for signaling, or exogenously by way of genetically encoded chemical crosslinkers (GECX) for finding out elusive protein-protein interactions. However, it stays difficult to establish these protein crosslinks effectively on the proteomic scale. Herein, software program OpenUaa is developed for figuring out protein crosslinks generated by genetically encoded unnatural amino acids and endogenous protein conjugation. OpenUaa options inclusive and open search functionality, dramatically enhancing identification sensitivity and protection.

Integrating GECX with OpenUaa, the direct interactome of thioredoxin is recognized in Escherichia coli cells, yielding 289 crosslinked peptides and similar to 205 direct binding protein of thioredoxin. These recognized direct binders present proof for thioredoxin’s regulation of redox state and mitochondria power metabolism. When figuring out endogenous conjugation of small ubiquitin-like modifier (SUMO), OpenUaa additionally markedly improves protection of SUMOylated peptides by ≈92%, revealing new SUMO targets. GECX-OpenUaa will allow environment friendly identification of direct interactomes of varied proteins in dwell cells.

Synaptic transmission depends on the continuous exocytosis and recycling of synaptic vesicles. Aged vesicle proteins are prevented from recycling and are ultimately degraded. This implies that lively synapses would lose vesicles and vesicle-associated proteins over time, except the provision correlates to exercise, to steadiness the losses. To check this speculation, we first mannequin the quantitative relation between presynaptic spike price and vesicle turnover. The mannequin predicts that the vesicle provide wants to extend with the spike price. To observe up this prediction, we measure protein turnover in particular person synapses of cultured hippocampal neurons by combining nanoscale secondary ion mass spectrometry (nanoSIMS) and fluorescence microscopy.

Design of Abiological Digital Poly(phosphodiester)s

Fast Nanoliter-Scale Cell Assays Using Droplet Microarray-Mass Spectrometry Imaging

In pharmaceutical analysis and growth, cell-based assays are primarily used with readout that depend on fluorescence-based and different label-dependent methods for evaluation of totally different mobile processes. Superhydrophobic-hydrophilic droplet microarrays (DMA) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) have lately emerged as key applied sciences for miniaturized high-throughput cell assays and for label-free molecular high-content drug profiling, respectively. Here, nanoliter-scale cell assays are built-in on DMAs with MALDI-MS imaging (MALDI-MSI) approaches to a droplet microarray-mass spectrometry imaging (DMA-MSI) platform.

Pancreas/duodenum Homeobox Protein 1 (PDX1) Antibody

abx033651-400ul 400 ul
EUR 523

Pancreas/duodenum Homeobox Protein 1 (PDX1) Antibody

abx033651-80l 80 µl
EUR 286

Pancreas/duodenum Homeobox Protein 1 (PDX1) Antibody

abx015963-100ug 100 ug
EUR 411

Pancreas/duodenum Homeobox Protein 1 (PDX1) Antibody

abx015964-100ug 100 ug
EUR 411

Pancreas/duodenum homeobox protein 1 (PDX1) Antibody

20-abx241830
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Pancreas/duodenum homeobox protein 1 (PDX1) Antibody

20-abx242867
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Pancreas/duodenum homeobox protein 1 (PDX1) Antibody

20-abx213983
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Pancreas/duodenum homeobox protein 1 (PDX1) Antibody

20-abx327762
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Mouse Pancreas/duodenum homeobox protein 1 (PDX1) ELISA Kit

abx520945-96tests 96 tests
EUR 668

Rat Pancreas/duodenum homeobox protein 1 (PDX1) ELISA Kit

abx520946-96tests 96 tests
EUR 668

Rat Pdx1/ Pancreas/duodenum homeobox protein 1 ELISA Kit

E0744Ra 1 Kit
EUR 646

Human PDX1/ Pancreas/duodenum homeobox protein 1 ELISA Kit

E1899Hu 1 Kit
EUR 605

Human Pancreas/duodenum homeobox protein 1 (PDX1) ELISA Kit

abx251794-96tests 96 tests
EUR 668

ELISA kit for Human Pancreas/duodenum homeobox protein 1

EK4887 96 tests
EUR 603
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Pancreas/duodenum homeobox protein 1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human PDX1(Pancreas/duodenum homeobox protein 1) ELISA Kit

EH2431 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Human Pancreas/duodenum homeobox protein 1, PDX1 ELISA KIT

ELI-15305h 96 Tests
EUR 824

Mouse Pancreas/duodenum homeobox protein 1, Pdx1 ELISA KIT

ELI-16116m 96 Tests
EUR 865

Recombinant (E.Coli, His-tag) Human Pancreas S Duodenum Homeobox-1

RP-864 10 ug
EUR 225

Pdx1 ELISA Kit| Mouse Pancreas/duodenum homeobox protein 1 ELIS

EF015784 96 Tests
EUR 689

Pdx1 ELISA Kit| Rat Pancreas/duodenum homeobox protein 1 ELISA

EF019128 96 Tests
EUR 689

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Rabbit anti-sEH

SEH-1 50 uL
EUR 280

Anti-Homeobox protein SIX6 Antibody

A06502-1 100ug/vial
EUR 294

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Motor Neuron And Pancreas Homeobox Protein 1 (MNX1) Antibody

abx028845-400ul 400 ul
EUR 523

Motor Neuron And Pancreas Homeobox Protein 1 (MNX1) Antibody

abx028845-80l 80 µl
EUR 286

Motor Neuron And Pancreas Homeobox Protein 1 (MNX1) Antibody

20-abx216901
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Motor neuron and pancreas homeobox protein 1 (MNX1) Antibody

20-abx338605
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Duodenum Lysate

1364 0.1 mg
EUR 191
Description: Duodenum tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

CK PANCREAS POWDER LYOPH 10g*

43032-1 10g
EUR 333.61

Motor neuron and pancreas homeobox protein 1 (MNX1) Antibody (HRP)

20-abx336588
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Motor neuron and pancreas homeobox protein 1 (MNX1) Antibody (FITC)

20-abx336589
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Motor neuron and pancreas homeobox protein 1 (MNX1) Antibody (Biotin)

20-abx336590
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Paraffin Tissue Section - Human Small Intestine Tumor: Duodenum Adenocarcinoma

T2235226-1 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Duodenum Tumor Lysate

1328 0.1 mg
EUR 254
Description: Duodenum tumor tissue lysate was prepared by homogenization in lysis buffer (50 mM Tris-HCl pH7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1% Triton x-100, 1% DOC, 0.1% SDS). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Duodenum Membrane Lysate

XBL-10547 0.1 mg
EUR 516.5
Description: Human duodenum tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human duodenum tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated duodenum tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated duodenum tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Mouse Motor neuron and pancreas homeobox protein 1 (MNX1) ELISA Kit

abx516940-96tests 96 tests
EUR 739

Mouse Mnx1/ Motor neuron and pancreas homeobox protein 1 ELISA Kit

E0966Mo 1 Kit
EUR 632

Human MNX1/ Motor neuron and pancreas homeobox protein 1 ELISA Kit

E1625Hu 1 Kit
EUR 605

Human Motor neuron and pancreas homeobox protein 1 (MNX1) ELISA Kit

abx250899-96tests 96 tests
EUR 739

ELISA kit for Human Motor neuron and pancreas homeobox protein 1

EK3406 96 tests
EUR 670
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Motor neuron and pancreas homeobox protein 1 in samples from serum, plasma, tissue homogenates and other biological fluids.

Human MNX1(Motor neuron and pancreas homeobox protein 1) ELISA Kit

EH1608 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 46.9pg/ml

Anti-Sumo 1 Rabbit Monoclonal Antibody

M00631-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Sumo 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Cytokeratin 1 Rabbit Monoclonal Antibody

M01639-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Cytokeratin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Paraffin Tissue Section - Human Pancreas Tumor: Adenocarcinoma

T2235188-1 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Rabbit PANCREAS Y 25 EA*

41231-2 25 EA
EUR 341.23

Rabbit PANCREAS MATURE 25 EA*

41331-2 25 EA
EUR 341.23

ELISA kit for Mouse Motor neuron and pancreas homeobox protein 1 (MNX1)

KTE70989-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Mouse Motor neuron and pancreas homeobox protein 1 (MNX1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Motor neuron and pancreas homeobox protein 1 (MNX1)

KTE70989-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Mouse Motor neuron and pancreas homeobox protein 1 (MNX1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Motor neuron and pancreas homeobox protein 1 (MNX1)

KTE70989-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Mouse Motor neuron and pancreas homeobox protein 1 (MNX1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Motor neuron and pancreas homeobox protein 1 (MNX1)

KTE61575-48T 48T
EUR 332
Description: Quantitative sandwich ELISA for measuring Human Motor neuron and pancreas homeobox protein 1 (MNX1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Motor neuron and pancreas homeobox protein 1 (MNX1)

KTE61575-5platesof96wells 5 plates of 96 wells
EUR 2115
Description: Quantitative sandwich ELISA for measuring Human Motor neuron and pancreas homeobox protein 1 (MNX1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Motor neuron and pancreas homeobox protein 1 (MNX1)

KTE61575-96T 96T
EUR 539
Description: Quantitative sandwich ELISA for measuring Human Motor neuron and pancreas homeobox protein 1 (MNX1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody

M00066-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal HMGB1/Hmg 1 Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-p27 KIP 1 Rabbit Monoclonal Antibody

M00173-1 100ug/vial
EUR 397
Description: Anti-p27 KIP 1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Rat

Anti-MHC class 1 Rabbit Monoclonal Antibody

M00194-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal MHC class 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human.

Anti-Integrin beta 1 Rabbit Monoclonal Antibody

M00772-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Integrin beta 1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody

M01106-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal ARG1/Arginase 1 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody

M02033-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal MLANA/Mart 1 Antibody. Validated in IF, WB and tested in Human.

Anti-DSG1/Desmoglein 1 Rabbit Monoclonal Antibody

M02655-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal DSG1/Desmoglein 1 Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Polyclonal Goat Anti-Homeobox B13 Antibody

APG00160G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-Homeobox B13 . This antibody is tested and proven to work in the following applications:

Human Duodenum Tumor lysate

HTL-1328 1 mg
EUR 895

Duodenum Diabetic Disease Lysate

XBL-10336 0.1 mg
EUR 663.5
Description: Human duodenum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human duodenum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the duodenum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The duodenum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Duodenum Liver Cirrhosis Lysate

XBL-10337 0.1 mg
EUR 663.5
Description: Human duodenum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human duodenum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the duodenum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The duodenum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Anti-CETP Antibody (polyclonal rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody

EXOAB-CETP-1 25 ul
EUR 219

Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody

M00522-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal AFP/Alpha 1 Fetoprotein Antibody. Validated in IP, IF, WB and tested in Human.

Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody

M00720-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal SERPINA1/Alpha 1 Antitrypsin Antibody. Validated in IP, IF, WB and tested in Human.

Anti-GPX1/Glutathione Peroxidase 1 Rabbit Monoclonal Antibody

M01019-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal GPX1/Glutathione Peroxidase 1 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

Anti-BAG-1 Rabbit Monoclonal Antibody, Clone#RM356

M02423-1 100uL
EUR 385
Description: Anti-BAG-1 Rabbit Monoclonal Antibody, Clone#RM356 tested in WB, IHC, reactive to Human

Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378

M04431-1 100uL
EUR 385
Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human

Pancreas Lysate

1307 0.1 mg
EUR 191
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lysate

1414 0.1 mg
EUR 191
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Pancreas Lysate

1469 0.1 mg
EUR 191
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Anti-Phospho-AMPK alpha 1 (S496) Rabbit Monoclonal Antibody

P00994-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Phospho-AMPK alpha 1 (S496) Antibody. Validated in IP, IF, WB and tested in Human.

Anti-p53 Rabbit Monoclonal Antibody

M00001-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal p53 Antibody. Validated in IP, IF, WB and tested in Human.

Anti-PTEN Rabbit Monoclonal Antibody

M00006-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal PTEN Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-STAT3 Rabbit Monoclonal Antibody

M00007-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal STAT3 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-BDNF Rabbit Monoclonal Antibody

M00035-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal BDNF Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-STAT1 Rabbit Monoclonal Antibody

M00036-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal STAT1 Antibody. Validated in IP, WB and tested in Human, Mouse.

Anti-VEGF Rabbit Monoclonal Antibody

M00045-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal VEGF Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC and tested in Human, Mouse.

Anti-CD44 Rabbit Monoclonal Antibody

M00052-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal CD44 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse.

Anti-Insulin Rabbit Monoclonal Antibody

M00067-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Insulin Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-Rad51 Rabbit Monoclonal Antibody

M00088-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Rad51 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Smad2 Rabbit Monoclonal Antibody

M00090-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Smad2 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-Tau Rabbit Monoclonal Antibody

M00097-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Tau Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-SOX2 Rabbit Monoclonal Antibody

M00105-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal SOX2 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse.

Anti-Src Rabbit Monoclonal Antibody

M00107-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Src Antibody. Validated in IF, WB and tested in Human, Rat.

Anti-PCNA Rabbit Monoclonal Antibody

M00125-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal PCNA Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-CD11b Rabbit Monoclonal Antibody

M00144-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal CD11b Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse.

Anti-SHP2 Rabbit Monoclonal Antibody

M00150-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal SHP2 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat.

Anti-CD19 Rabbit Monoclonal Antibody

M00154-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal CD19 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-CDK4 Rabbit Monoclonal Antibody

M00159-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal CDK4 Antibody. Validated in IF, WB and tested in Human.

Anti-p63 Rabbit Monoclonal Antibody

M00167-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal p63 Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

Anti-PD1 Rabbit Monoclonal Antibody

M00178-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal PD1 Antibody. Validated in IHC and tested in Human.

Using A549 lung most cancers cells, concentration-response profiling of a pharmaceutical compound, the fatty acid synthase inhibitor GSK2194069, are demonstrated. Direct cell tradition on DMAs permits mixture of microscopy and excessive velocity, excessive molecular content material evaluation utilizing MALDI-MSI. Miniaturization of array spots all the way down to 0.5 mm confining 40 nL droplets permits for MALDI imaging evaluation of as few as ten cells per spot. Partial automation ensures a quick pattern preparation workflow. Taken collectively, the built-in DMA-MSI platform that mixes MALDI-MSI, as a label-free analytical readout, with the miniaturized droplet microarray platform is a worthwhile complement to excessive throughput cell-based assays applied sciences.