Lung most cancers has the next incidence fee and mortality fee than all different cancers. Early prognosis and therapy of lung most cancers stay a serious problem, and the 5-year survival fee of its sufferers is barely 15%. Basic and scientific analysis, particularly the invention of biomarkers, is essential for enhancing the prognosis and therapy of lung most cancers sufferers. To establish novel biomarkers for lung most cancers, we used the iTRAQ8-plex labeling expertise mixed with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to investigate the serum and urine of sufferers with totally different phases of lung adenocarcinoma and wholesome people. A complete of 441 proteins had been recognized within the serum, and 1,161 proteins had been recognized within the urine.
The ranges of elongation issue 1-alpha 2, proteasome subunit alpha kind, and spermatogenesis-associated protein elevated considerably within the serum of sufferers with lung most cancers in contrast with these in wholesome controls. The ranges of transmembrane protein 143, cadherin 5, fibronectin 1, and collectin-11 decreased considerably within the serum of sufferers with metastases in contrast with these of nonmetastatic lung most cancers sufferers. In the urine of stage III and IV lung most cancers sufferers, the prostate-specific antigen and prostatic acid phosphatase decreased considerably, whereas neutrophil defensin 1 elevated considerably
. The outcomes of LC-MS/MS had been confirmed by enzyme-linked immunosorbent assay (ELISA) for transmembrane protein 143, cadherin 5, fibronectin 1, and collectin-11 within the serum. These proteins could also be a possible early prognosis and metastasis biomarkers for lung adenocarcinoma. Furthermore, the relative content material of these markers within the serum and urine could possibly be used to find out the development of lung adenocarcinoma and obtain correct staging and prognosis.
Integrated Screens Identify CDK1 as a Therapeutic Target in Advanced Gastrointestinal Stromal Tumors
Oncogenic KIT or PDGFRA receptor tyrosine kinase mutations are compelling therapeutic targets in gastrointestinal stromal tumor (GIST), and therapy with the KIT/PDGFRA inhibitor imatinib is the usual of take care of sufferers with superior GIST. Polyclonal emergence of KIT/PDGFRA secondary mutations is the primary mechanism of imatinib development, making it difficult to beat KIT/PDGFRA-inhibitor resistance. It is unclear whether or not there are different therapeutic targets in superior GIST. Using genome-wide transcriptomic profiling of superior versus early-stage GIST and CRISPR knockout useful screens, we exhibit that CDK1 is often extremely expressed in superior GIST however not in early-stage GIST throughout three affected person cohorts. High expression of CDK1 was related to malignancy in GIST.
CDK1 was critically required for superior GIST, together with imatinib-resistant GIST. CDK1 ablation led to strong proliferation inhibition. A mass spectrometry-based proteomics display screen additional revealed that AKT is a novel substrate of CDK1 kinase in GIST. CDK1 certain AKT and controlled its phosphorylation, thereby selling GIST proliferation and development. Importantly, a pharmacological inhibitor of CDK1, RO-3306, disrupted GIST cell proliferation in CDK1 extremely expressed GIST however not in CDK1-negative GIST cells and non-transformed fibroblast cells.
Treatment with RO-3306 diminished tumor progress in each imatinib-resistant and imatinib-sensitive GIST xenograft mouse fashions. Our findings counsel that CDK1 represents a druggable therapeutic goal in GIST and warrants additional testing in scientific trials. Significant distinction in expression was noticed between vulnerable scientific and MDR strains, in addition to vulnerable scientific and environmental strains. Transition from an environmental saprophyte to a scientific pressure might alter its physiological traits to additional enhance its adaptation. This made it potential to enhance the selectivity within the willpower of these compounds in comparison with that obtained with conventional GC-MS ion sources.
Here, a brand new fuel chromatography-atmospheric strain photoionization-high-resolution mass spectrometry (GC-APPI-HRMS) technique mixed with selective pressurized liquid extraction (sPLE) has been developed for the selective willpower of Dechlorane Plus (DP) and its associated compounds in gull egg samples used as a bioindicator of contamination. To one of the best of our data, that is the primary time these compounds have been analyzed by GC-MS utilizing atmospheric strain photoionization (APPI). Negative ion dopant-assisted APPI utilizing vapors of diethyl ether and a supply temperature of 250 °C supplied excessive ionization efficiencies and mass spectra characterised by intense in-source fragment ions in addition to the presence of molecular ion and attribute cluster ions containing oxygen atoms of their chemical construction.
Proteomic profiling of scientific and environmental strains of Pseudomonas aeruginosa
Pseudomonas aeruginosa is a ubiquitous bacterium, which is ready to change its physiological traits in response to totally different habitats. Environmental strains are presumably much less pathogenic than scientific strains and whether or not or not the scientific strains originate from the atmosphere or by way of inter-host transmission stays poorly understood. To reduce the danger of an infection, a greater understanding of proteomic profiling of P. aeruginosa is critical for elucidating the correlation between environmental and scientific strains. Based on antimicrobial susceptibility and patterns of virulence, we chosen 12 scientific and environmental strains: (i) environmental, (ii) multidrug resistant (MDR) scientific and (iii) vulnerable scientific strains.
Mammalian Total Protein Extraction Kit |
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| 20-abx098853 | Abbexa |
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Total Protein Extraction Kit for Tendons (50 tests) |
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| P5A04 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Muscles (50 tests) |
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| P5A06 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Insects (50 tests) |
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| P5A07 | 101Bio | - | Ask for price |
Total RNA Extraction Kit |
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| K2014005 | Biochain | 1 kit | EUR 344 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Total Protein Extraction Kit for Skin Tissue (50 tests) |
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| P5A01 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Bone Tissue (50 tests) |
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| P5A02 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Blood Vessels (50 tests) |
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| P5A03 | 101Bio | - | Ask for price |
Total Protein Extraction Kit: TM Buffer |
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| K3011010-1 | Biochain | 13 ml | EUR 162 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Total Protein Extraction Kit: 50X PI |
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| K3011010-2 | Biochain | 260 ul | EUR 289 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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EpiQuik Total Histone Extraction Kit |
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| OP-0006 | EpiGentek | 100 Extractions | EUR 225.45 |
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Description: The best epigenetics products |
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Xpert Total Histone Extraction Kit |
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| H7200-050 | GenDepot | 50 ext | EUR 361 |
Xpert Total Histone Extraction Kit |
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| H7200-100 | GenDepot | 100 ext | EUR 509 |
AnaPrep Total RNA Extraction Kit |
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| Z1322015 | Biochain | 1 kit (48 extractions) Including all required plastic disposables | EUR 308 |
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Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications. |
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AffiSelect Total Protein Extraction Solution |
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| A0710-015 | GenDepot | 15X1ml | EUR 142 |
Bacterial Total Protein Extraction Reagent |
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| abx090632-50100assays | Abbexa | 50-100 assays | EUR 258 |
Plant Total Protein Extraction Reagent |
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| abx090633-50100assays | Abbexa | 50-100 assays | EUR 230 |
Total Protein Extraction Kit for Adipose Tissues and Cultured Adipocytes |
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| P522-20 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Adipose Tissues and Cultured Adipocytes |
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| P522-4 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Adipose Tissues and Cultured Adipocytes |
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| P522-80 | 101Bio | - | Ask for price |
Total Protein Extraction Kit for Intestines and Mesentery (50 tests) |
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| P5A05 | 101Bio | - | Ask for price |
Human Total PSA (t-PSA) ELISA Kit |
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| PRB-5049-TOTAL | Cell Biolabs | 96 assays | EUR 572 |
3-min Bacterial Total Protein Extraction Kit |
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| P512L | 101Bio | - | Ask for price |
Trypin for Mass & Sequencing |
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| T9600-025 | GenDepot | 25ug | EUR 145 |
Trypin for Mass & Sequencing |
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| T9600-100 | GenDepot | 100ug | EUR 219 |
Trypin for Mass & Sequencing |
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| T9600-112 | GenDepot | 12x100ug | EUR 1657 |
Trypin for Mass & Sequencing |
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| T9600-400 | GenDepot | 4x100ug | EUR 651 |
EpiQuik Total Histone Extraction HT Kit |
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| OP-0007 | EpiGentek | 192 Extractions | EUR 395.1 |
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Description: reagents widely cited |
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Rapid Animal Total RNA Extraction Kit |
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| AT4181 | Bio Basic | 50Preps | EUR 88.06 |
Rapid Animal Total RNA Extraction Kit |
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| AT4182 | Bio Basic | 250preps | EUR 202.25 |
3-min Total Protein Extraction Kit (Animal cells) |
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| P501 | 101Bio | - | Ask for price |
3-min Total Protein Extraction Kit (Animal cells) |
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| P501L | 101Bio | - | Ask for price |
3-min Total Protein Extraction Kit (Animal cells) |
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| P501S | 101Bio | - | Ask for price |
3-min Total Protein Extraction Kit (Animal Tissues) |
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| P502 | 101Bio | - | Ask for price |
3-min Total Protein Extraction Kit (Animal Tissues) |
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| P502L | 101Bio | - | Ask for price |
3-min Total Protein Extraction Kit (Animal Tissues) |
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| P502S | 101Bio | - | Ask for price |
8-min Plant Tissue Total Protein Extraction Kit |
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| P508L | 101Bio | - | Ask for price |
Extract-EZ E1, Total Subcellular Protein Extraction Kit |
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| BSP073 | Bio Basic | 50Preps | EUR 135.26 |
Human Total PSA (t-PSA) ELISA Kit |
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| PRB-5049-TOTAL-5 | Cell Biolabs | 5 x 96 assays | EUR 2283 |
RNA isolater Total RNA Extraction Reagent |
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| R401-01 | Vazyme | 100 ml | EUR 133 |
AnaPrep Total RNA Extraction Kit with DNase-Treatment |
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| Z1322017 | Biochain | 1 kit (48 extractions) Including all required plastic disposables | EUR 405 |
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Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications. |
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3-min Detergent-free Total Protein Extraction Kit (Animal Cells) |
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| P505 | 101Bio | - | Ask for price |
3-min Detergent-free Total Protein Extraction Kit (Animal Cells) |
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| P505L | 101Bio | - | Ask for price |
3-min Detergent-free Total Protein Extraction Kit (Animal Cells) |
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| P505S | 101Bio | - | Ask for price |
3-min Detergent-free Total Protein Extraction Kit (Animal Tissues) |
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| P506 | 101Bio | - | Ask for price |
3-min Detergent-free Total Protein Extraction Kit (Animal Tissues) |
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| P506L | 101Bio | - | Ask for price |
3-min Detergent-free Total Protein Extraction Kit (Animal Tissues) |
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| P506S | 101Bio | - | Ask for price |
8-min Plant Tissue Detergent-free Total Protein Extraction Kit |
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| P510L | 101Bio | - | Ask for price |
10-min. Thick Cell Wall Microbes Total Protein Extraction Kit |
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| P515-50 | 101Bio | - | Ask for price |
Triton Extraction Buffer(TEB), for Histon Extraction |
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| T8300-100 | GenDepot | 100ml | EUR 126 |
Triton Extraction Buffer(TEB), for Histon Extraction |
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| T8300-500 | GenDepot | 500ml | EUR 142 |
EZ-10 Spin Column Viral Total RNA Extraction Kit |
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| VT82112 | Bio Basic | 50Preps | EUR 203.77 |
Total RNA Extraction Kit (Proteinase K & DNase I included) |
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| TR05 | GeneOn | 5 preps | Ask for price |
Total RNA Extraction Kit (Proteinase K & DNase I included) |
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| TR100 | GeneOn | 100 preps | EUR 315 |
Total RNA Extraction Kit (Proteinase K & DNase I included) |
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| TR25 | GeneOn | 25 preps | EUR 147 |
Total RNA Extraction Kit (Proteinase K & DNase I included) |
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| TR50 | GeneOn | 50 preps | EUR 211 |
Total Exosome RNA Extraction Kit (Biological Fluids/Culture Media Supplement) |
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| EIRK-03 | Creative Biolabs | 20 reactions | EUR 2817 |
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Description: This product is a useful tool for the isolation and efficient total RNA (miRNA and mRNAs) extraction from the total exosome population from human biofluids or cell culture media. |
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Blood Total RNA Extraction Kit (Proteinase K & DNase I included) |
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| TB05 | GeneOn | 5 preps | Ask for price |
Blood Total RNA Extraction Kit (Proteinase K & DNase I included) |
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| TB25 | GeneOn | 25 preps | EUR 147 |
Plant Protein Extraction Kit |
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| BSP004 | Bio Basic | 20Preps | EUR 110.9 |
Nuclear Protein Extraction Kit |
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| BSP009 | Bio Basic | 50Preps | EUR 89.15 |
FFPE Protein Extraction Kit |
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| BSP058 | Bio Basic | 50Preps | EUR 102.2 |
Membrane Protein Extraction Kit |
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| K2113-50 | ApexBio | 50 assays | EUR 544 |
Membrane Protein Extraction Kit |
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| K268-50 | Biovision | EUR 539 | |
Membrane Protein Extraction Kit |
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| K3014005 | Biochain | 1 kit | EUR 382 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Membrane Protein Extraction Kit |
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| 55R-1357 | Fitzgerald | 50 assays | EUR 740 |
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Description: Membrane Protein Extraction Kit for use in the research laboratory |
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DNA Extraction Buffer for wells |
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| D105 | Cygnus Technologies | 30 ml | EUR 204 |
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Description: DNA Extraction Buffer for wells by Cygnus Technologies is available in Europe via Gentaur. |
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DNA Extraction Buffer for tubes |
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| D108 | Cygnus Technologies | 30 ml | EUR 204 |
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Description: DNA Extraction Buffer for tubes by Cygnus Technologies is available in Europe via Gentaur. |
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Viral RNA Extraction Kit for Respiratory Specimens |
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| K1462-250 | Biovision | 250 Rxns | EUR 1006 |
Viral RNA Extraction Kit for Respiratory Specimens |
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| K1462-50 | Biovision | 50 Rxns | EUR 399 |
Protein Extraction for Fixed and Embedded Tissues |
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| P525-20 | 101Bio | - | Ask for price |
Protein Extraction Kit for Hair, Nail, Wool and Horn |
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| P521-20 | 101Bio | - | Ask for price |
Protein Extraction Kit for Hair, Nail, Wool and Horn |
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| P521-4 | 101Bio | - | Ask for price |
Protein Extraction Kit for Hair, Nail, Wool and Horn |
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| P521-80 | 101Bio | - | Ask for price |
ExoDNA? Extraction Kit |
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| K1242-25 | Biovision | EUR 588 | |
ExoDNA? Extraction Kit |
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| K1242-50 | Biovision | EUR 881 | |
miRNA Extraction Kit |
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| K1456-50 | Biovision | 50 Preps | EUR 551 |
Xpert Plant Protein Extraction Kit |
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| P3113-050 | GenDepot | 50 Prep | EUR 550 |
Prolytic Membrane Protein Extraction Kit |
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| P6300-020 | GenDepot | 20 prep | EUR 266 |
Prolytic Membrane Protein Extraction Kit |
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| P6300-050 | GenDepot | 50 prep | EUR 469 |
Serum Exosomal Protein Extraction kit |
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| P300 | 101Bio | - | Ask for price |
Serum Exosomal Protein Extraction kit |
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| P300S | 101Bio | - | Ask for price |
Inclusion Body Protein Extraction Kit |
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| BS686 | Bio Basic | 1kit, 10prep | EUR 106.55 |
Mammalian Membrane Protein Extraction Kit |
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| AR0155 | BosterBio | 1 kit | EUR 152 |
Mammalian Membrane Protein Extraction Kit |
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| 20-abx098855 | Abbexa |
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CNM Compartmental Protein Extraction Kit |
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| K3012010 | Biochain | 1 kit | EUR 412 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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CNMCS Compartmental Protein Extraction Kit |
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| K3013010 | Biochain | 1 kit | EUR 496 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Miniprep Kit For Plasmid DNA Extraction And Purification |
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| MB1005-100Reactions | BosterBio | 100 Reactions | EUR 143 |
Miniprep Kit For Plasmid DNA Extraction And Purification |
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| MB1005-250Reactions | BosterBio | 250 Reactions | EUR 259 |
Miniprep Kit For Plasmid DNA Extraction And Purification |
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| MB1005-50Reactions | BosterBio | 50 Reactions | EUR 120 |
AnaPrep HPV DNA Extraction Kit for swab samples |
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| Z1322007 | Biochain | 1 kit (48 extractions) Including all required plastic disposables | EUR 276 |
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Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications. |
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ELISA kit for Rat Total Carnitine |
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| KTE100967-48T | Abbkine | 48T | EUR 354 |
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Description: Quantitative sandwich ELISA for measuring Rat Total Carnitine in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Total Carnitine |
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| KTE100967-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2252 |
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Description: Quantitative sandwich ELISA for measuring Rat Total Carnitine in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Rat Total Carnitine |
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| KTE100967-96T | Abbkine | 96T | EUR 572 |
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Description: Quantitative sandwich ELISA for measuring Rat Total Carnitine in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Total IgE |
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| KTE62846-48T | Abbkine | 48T | EUR 354 |
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Description: Quantitative sandwich ELISA for measuring Human Total IgE in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Total IgE |
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| KTE62846-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2252 |
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Description: Quantitative sandwich ELISA for measuring Human Total IgE in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Total IgE |
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| KTE62846-96T | Abbkine | 96T | EUR 572 |
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Description: Quantitative sandwich ELISA for measuring Human Total IgE in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Mouse Legumain (total) |
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| EK5744 | SAB | 96 tests | EUR 553 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Legumain (total) in samples from serum, plasma, tissue homogenates and other biological fluids. |
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Whole-cell protein was extracted from every pressure and subjected to two-dimensional differential gel electrophoresis (2-D DIGE) and liquid chromatography tandem mass spectrometry quadrupole time-of-flight (LC-MS QTOF). All 12 strains had been clustered into three distinct teams primarily based on their variance in protein expression. A complete of 526 matched spots had been detected and 4 differentially expressed protein spots (p < 0.05) had been recognized and all differential spots had been downregulated in MDR pressure J3. Upregulation of chitin binding and BON area proteins was current within the environmental and a few MDR strains, whereas the scientific strains exhibited distinct proteomic profiles with elevated expression of serine protein kinase and arginine/ornithine transport ATP-binding proteins.