The noncanonical heme oxygenase MhuD from Mycobacterium tuberculosis binds a heme substrate that adopts a dynamic equilibrium between planar and out-of-plane ruffled conformations. MhuD degrades this substrate to an uncommon mycobilin product through successive monooxygenation and dioxygenation reactions. This article establishes a causal relationship between heme substrate dynamics and MhuD-catalyzed heme degradation, leading to a refined enzymatic […]
Category: Medium & Serums
Rapid Denaturing Organic Digestion Method for Targeted Protein Identification and Characterization
Bottom-up mass spectrometry-based protein evaluation strategies using protease digestion are routinely used to establish and characterize proteins with excessive specificity and sensitivity. Method efficiency is usually measured by sequence protection functionality and the entire variety of attribute peptides recognized, when in comparison with predicted databases. Limitations to generally used solvent-based digestion strategies at present employed embody lengthy […]