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Rapid Denaturing Organic Digestion Method for Targeted Protein Identification and Characterization
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Rapid Denaturing Organic Digestion Method for Targeted Protein Identification and Characterization

- March 18, 2021 | March 19, 2021 - Barry Alexander

Bottom-up mass spectrometry-based protein evaluation strategies using protease digestion are routinely used to establish and characterize proteins with excessive specificity and sensitivity. Method efficiency is usually measured by sequence protection functionality and the entire variety of attribute peptides recognized, when in comparison with predicted databases. Limitations to generally used solvent-based digestion strategies at present employed embody lengthy digestion occasions (18-24 h or extra), resulting in protease autolysis, which additionally precludes automation, decreases sensitivity, and will increase each intra- and inter-day efficiency variability.

This report describes the event and validation of a easy, 5 min tryptic denaturing natural digestion (DOD) technique for use with tandem mass spectrometry in bottom-up protein identification and characterization. It has been evaluated throughout choose protein toxins and diagnostic scientific protein targets, considerably bettering digestion efficiency when in comparison with different solution-based and enzyme-immobilized strategies. The technique was in comparison with two at present used bottom-up strategies, the 24 h filter-aided pattern prep (FASP) and Flash Digest (1 and Four h) strategies.

Single proteins used to match the strategies included the ricin gentle chain, ricin heavy chain, ricin holotoxin, serotype A Clostridium botulinum toxin, Staphylococcus enterotoxin B, ribonuclease A, and thyroglobulin. In checks, throughout the proteins investigated, the 5 min DOD digestion technique resulted in sequence coverages starting from 55 to 100%, with comparatively excessive reproducibility and precision; outcomes have been higher than or equal to FASP technique outcomes and have been tremendously enhanced when in comparison with Flash technique outcomes.

Importantly, DOD technique intra- and inter-day precision was a lot improved as in comparison with outcomes for each FASP and Flash digestions. These knowledge indicated that the DOD technique, when in comparison with the FASP and Flash Digest strategies, dramatically diminished digestion time, whereas sustaining or bettering the flexibility to detect and characterize focused proteins, and diminished analytical variability for tryptic digestion, leading to markedly quicker and extra exact analyses.

The urinary glucose tetrasaccharide, Glcα1-6Glcα1-4Glcα1-4Glc (Glc4), is a glycogen restrict dextrin that’s elevated in sufferers with glycogen storage illness (GSD) kind III. We evaluated the potential of raw cornstarch remedy to intervene with Glc4 monitoring, by measuring the diurnal variability of Glc4 excretion in sufferers with GSD III. PLHIV reported moderate-severe ache had greater hair cortisone than these reported gentle (p=0.070) or none ache (p=0.014), with no variations between the latter two ache severity teams. Greater ache severity is related to greater hair cortisone ranges amongst Chinese PLHIV.
Voids have been collected at dwelling over 24 hours, saved at 4°C and frozen inside 48 hours. Glc4 was analyzed utilizing liquid chromatography-tandem mass spectrometry and normalized to creatinine. Subjects with GSD III (median age: 13.5 years, vary: 3.7-62; n = 18) accomplished a number of 24-hour urine assortment, and 28/36 collections have been accepted for evaluation. Glc4 was elevated in 16/18 topics (median: 13 mmol/mol creatinine, vary: 2-75, reference vary: <3). In collections with elevated Glc4 (23/28), two-thirds (15/23) had low diurnal variability in Glc4 excretion (coefficient of variation [CV%] <25). The diurnal variability was considerably correlated with the Glc4 focus (Pearson R = .644, P < .05), however not with the dose of raw cornstarch. High intraday variability (>25%) was not persistently noticed in repeat collections by the identical topic.
Rapid Denaturing Organic Digestion Method for Targeted Protein Identification and Characterization

Greater Pain Severity is Associated with Higher Glucocorticoid Levels in Hair Among a Cohort of People Living with HIV

Pain is a standard prevalence and persistent symptom, which has an antagonistic influence on particular person well-being and high quality of life amongst individuals dwelling with HIV (PLHIV). Alteration within the exercise of the Hypothalamic-Pituitary-Adrenal (HPA) axis leading to irregular glucocorticoid ranges had been proposed to play vital roles in these associations. This research aimed to analyze whether or not ache severity was related to hair glucocorticoid ranges, a novel technique of measuring long-term glucocorticoid publicity, amongst a big cohort of Chinese PLHIV.
A measure of ache severity and hair samples have been collected from 431 adults PLHIV in Guangxi, China. Glucocorticoid (cortisol and cortisone) in hair have been quantified by liquid chromatography-tandem mass spectrometry. The basic linear mannequin was used to check the associations of ache severity with hair glucocorticoid ranges after adjusting for potential confounding elements. Of the 431 PLHIV, 273 reported none ache, 87 reported gentle ache, and 71 reported moderate-severe ache. Hair cortisone, however not hair cortisol, was discovered to vary considerably among the many three ache severity teams (F=3.90, p=0.021).  In order to cut back the long-term glucocorticoid ranges, interventions managing ache must be thought of for PLHIV with moderate-severe ache.

Isotope sample deconvolution as a profitable various to calibration curve for software in wastewater-based epidemiology

An isotope sample deconvolution (IPD) quantification technique has been utilized for the willpower of 5 substances (amphetamine, benzoylecgonine, cocaine, methamphetamine and MDMA) in wastewater for the applying in wastewater-based epidemiology (WBE). A beforehand validated technique that used a calibration curve for quantification was modified to use IPD. The two approaches have been in contrast by way of analytical uncertainty in restoration research of high quality management samples, i.e. six wastewater samples from completely different geographical origins spiked at two focus ranges.

Both strategies have been dependable as they handed (z-score < 2) in an interlaboratory train. After 60 particular person determinations, IPD offered 11 outcomes exterior restoration limits (70-120%) whereas the earlier technique produced 31 antagonistic outcomes. All imply values for IPD have been correct whereas 6 out of 10 outcomes confirmed RSD values greater than 30% or recoveries exterior limits when utilizing the previous technique. Moreover, the calculated technique bias for the latter doubles that of IPD, which, in flip, makes the mixed uncertainty (u(c)) a lot greater.

ASAH2 Rabbit Polyclonal Antibody
MBS9466418-01mL 0.1mL
EUR 390

ASAH2 Rabbit Polyclonal Antibody
MBS9466418-5x01mL 5x0.1mL
EUR 1610

Rabbit Polyclonal ASAH2 Antibody
TA306622 100 µg Ask for price

ASAH2 (C-term) rabbit polyclonal antibody
AP23470PU-N 50 µg Ask for price

Rabbit Polyclonal to Human ASAH2
MBS247760-005mg 0.05mg
EUR 715

Rabbit Polyclonal to Human ASAH2
MBS247760-5x005mg 5x0.05mg
EUR 3035

ASAH2 Polyclonal Antibody
A62106
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

ASAH2 Polyclonal Antibody
E-AB-14747-120uL 120uL
EUR 240
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-14747-200uL 200uL
EUR 399
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-14747-20uL 20uL
EUR 73
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-14747-60uL 60uL
EUR 143
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-10960-120uL 120uL
EUR 240
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-10960-200uL 200uL
EUR 399
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-10960-20uL 20uL
EUR 73
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-10960-60uL 60uL
EUR 143
Description: Unconjugated

ASAH2 Polyclonal Antibody
A72338 50 μl
EUR 408

ASAH2 Polyclonal Antibody
A72339 50 μl
EUR 408

ASAH2 Polyclonal Antibody
E-AB-62491-120uL 120uL
EUR 320
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-62491-200uL 200uL
EUR 530
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-62491-60uL 60uL
EUR 200
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-62491-each each Ask for price
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-64270-120uL 120uL
EUR 320
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-64270-200uL 200uL
EUR 530
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-64270-60uL 60uL
EUR 200
Description: Unconjugated

ASAH2 Polyclonal Antibody
E-AB-64270-each each Ask for price
Description: Unconjugated

ASAH2 Polyclonal Antibody
MBS9237936-01mL 0.1mL
EUR 415

ASAH2 Polyclonal Antibody
MBS9237936-5x01mL 5x0.1mL
EUR 1841

ASAH2 Polyclonal Antibody
MBS9127971-002mL 0.02mL
EUR 200

ASAH2 Polyclonal Antibody
MBS9127971-005mL 0.05mL
EUR 255

ASAH2 Polyclonal Antibody
MBS9127971-01mL 0.1mL
EUR 345

ASAH2 Polyclonal Antibody
MBS9127971-02mL 0.2mL
EUR 545

ASAH2 Polyclonal Antibody
MBS9127971-5x02mL 5x0.2mL
EUR 2265

ASAH2 Polyclonal Antibody
MBS9132489-002mL 0.02mL
EUR 200

ASAH2 Polyclonal Antibody
MBS9132489-005mL 0.05mL
EUR 255

ASAH2 Polyclonal Antibody
MBS9132489-01mL 0.1mL
EUR 345

ASAH2 Polyclonal Antibody
MBS9132489-02mL 0.2mL
EUR 545

ASAH2 Polyclonal Antibody
MBS9132489-5x02mL 5x0.2mL
EUR 2265

ASAH2 Polyclonal Antibody
MBS2524355-002mL 0.02mL
EUR 135

ASAH2 Polyclonal Antibody
MBS2524355-006mL 0.06mL
EUR 190

ASAH2 Polyclonal Antibody
MBS2524355-012mL 0.12mL
EUR 265

ASAH2 Polyclonal Antibody
MBS2524355-02mL 0.2mL
EUR 415

ASAH2 Polyclonal Antibody
MBS2524355-5x02mL 5x0.2mL
EUR 1835

ASAH2 Polyclonal Antibody
MBS2525351-002mL 0.02mL
EUR 135

ASAH2 Polyclonal Antibody
MBS2525351-006mL 0.06mL
EUR 190

ASAH2 Polyclonal Antibody
MBS2525351-012mL 0.12mL
EUR 265

ASAH2 Polyclonal Antibody
MBS2525351-02mL 0.2mL
EUR 415

ASAH2 Polyclonal Antibody
MBS2525351-5x02mL 5x0.2mL
EUR 1835

ASAH2 Polyclonal Antibody
MBS2541020-006mL 0.06mL
EUR 190

ASAH2 Polyclonal Antibody
MBS2541020-012mL 0.12mL
EUR 265

ASAH2 Polyclonal Antibody
MBS2541020-02mL 0.2mL
EUR 415

ASAH2 Polyclonal Antibody
MBS2541020-5x02mL 5x0.2mL
EUR 1835

ASAH2 Polyclonal Antibody
MBS2554645-006mL 0.06mL
EUR 190

ASAH2 Polyclonal Antibody
MBS2554645-012mL 0.12mL
EUR 265

ASAH2 Polyclonal Antibody
MBS2554645-02mL 0.2mL
EUR 415

ASAH2 Polyclonal Antibody
MBS2554645-5x02mL 5x0.2mL
EUR 1835

ASAH2 Polyclonal Antibody
RD77850A-120uL 120μL
EUR 360
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD77850A-200uL 200μL
EUR 630
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD77850A-20uL 20μL
EUR 109.5
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD77850A-60uL 60μL
EUR 214.5
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD75591A-120uL 120μL
EUR 360
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD75591A-200uL 200μL
EUR 630
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD75591A-20uL 20μL
EUR 109.5
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD75591A-60uL 60μL
EUR 214.5
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD81031A-120uL 120μL
EUR 360
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD81031A-200uL 200μL
EUR 630
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD81031A-60uL 60μL
EUR 204
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD84843A-120uL 120μL
EUR 360
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD84843A-200uL 200μL
EUR 630
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

ASAH2 Polyclonal Antibody
RD84843A-60uL 60μL
EUR 204
Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

Polyclonal ASAH2 Antibody
APG02010G 0.1 mg
EUR 790.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ASAH2 . This antibody is tested and proven to work in the following applications:

Polyclonal ASAH2 Antibody
APG02011G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ASAH2 . This antibody is tested and proven to work in the following applications:

Rabbit anti-Danio rerio (Zebrafish)(Brachydanio rerio) ASAH2 Polyclonal Antibody
MBS7196320-INQUIRE INQUIRE Ask for price

ASAH3 Rabbit Polyclonal Antibody
ES8356-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against ASAH3 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ASAH3 Rabbit Polyclonal Antibody
ES8356-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against ASAH3 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

ASAH3 Rabbit Polyclonal Antibody
E10G23389 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

ASAH1 Rabbit Polyclonal Antibody
E10G01084 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

ASAH1 Rabbit Polyclonal Antibody
E10G08026 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

ASAH3L Rabbit Polyclonal Antibody
ES8419-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against ASAH3L from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

ASAH3L Rabbit Polyclonal Antibody
ES8419-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against ASAH3L from Human/Mouse. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

ASAH3L Rabbit Polyclonal Antibody
E10G23259 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

ASAH2 Polyclonal Antibody, HRP Conjugated
A62107
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  • Ask for price
  • 100 µg
  • 50 ul
  • 100 ul

Rabbit Polyclonal ASAH1 Antibody
TA306621 100 µg Ask for price

ASAH2 Polyclonal Antibody, FITC Conjugated
A62108
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 µg
  • 50 ul
  • 100 ul

ASAH2 Polyclonal Antibody, Biotin Conjugated
A62109
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  • Ask for price
  • Ask for price
  • 100 µg
  • 50 ul
  • 100 ul

Rabbit polyclonal anti-ACER1 (ASAH3) antibody
TA316075 100 µl Ask for price

Rabbit anti-Mus musculus (Mouse) Asah1 Polyclonal Antibody
MBS9015055-INQUIRE INQUIRE Ask for price

Rabbit anti-Homo sapiens (Human) ASAH2B Polyclonal Antibody
MBS7138071-INQUIRE INQUIRE Ask for price

Rabbit anti-human Acid ceramidase polyclonal Antibody(ASAH1)
MBS1492517-005mL 0.05mL
EUR 190

Rabbit anti-human Acid ceramidase polyclonal Antibody(ASAH1)
MBS1492517-01mL 0.1mL
EUR 270

Rabbit anti-human Acid ceramidase polyclonal Antibody(ASAH1)
MBS1492517-5x01mL 5x0.1mL
EUR 1205

Rabbit anti-human Acid ceramidase polyclonal Antibody(ASAH1)
MBS1494769-005mL 0.05mL
EUR 190

Rabbit anti-human Acid ceramidase polyclonal Antibody(ASAH1)
MBS1494769-01mL 0.1mL
EUR 270

Rabbit anti-human Acid ceramidase polyclonal Antibody(ASAH1)
MBS1494769-5x01mL 5x0.1mL
EUR 1205

Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)
PAE689Hu01 100ul
EUR 245

Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)
PAE689Ra02 100ul
EUR 266

Rabbit anti-Caenorhabditis elegans asah-2 Polyclonal Antibody
MBS7144562-INQUIRE INQUIRE Ask for price

Rabbit anti-Caenorhabditis elegans asah-1 Polyclonal Antibody
MBS7138069-INQUIRE INQUIRE Ask for price

Rabbit anti-Pan troglodytes (Chimpanzee) ASAH1 Polyclonal Antibody
MBS7138070-INQUIRE INQUIRE Ask for price

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (IHC)
IRBAMLASAH2AP491120UL each
EUR 346
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (IHC)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (IHC)
IRBAMLASAH2AP491200UL each
EUR 509
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (IHC)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3)(IHC)
MBS8422577-INQUIRE INQUIRE Ask for price

Rabbit Polyclonal Anti-ASAH2 Antibody
TA349659 100 µl Ask for price

Rabbit anti Mouse ASAH2  Monoclonal Antibody
MBS2544428-006mL 0.06mL
EUR 250

Rabbit anti Mouse ASAH2  Monoclonal Antibody
MBS2544428-012mL 0.12mL
EUR 370

Rabbit anti Mouse ASAH2  Monoclonal Antibody
MBS2544428-02mL 0.2mL
EUR 550

Anti-ASAH2 Antibody, Rabbit Polyclonal
MBS8110750-01mL 0.1mL
EUR 300

Anti-ASAH2 Antibody, Rabbit Polyclonal
MBS8110750-5x01mL 5x0.1mL
EUR 1200

Anti-ASAH2 Antibody, Rabbit Polyclonal
MBS8110751-01mL 0.1mL
EUR 300

Anti-ASAH2 Antibody, Rabbit Polyclonal
MBS8110751-5x01mL 5x0.1mL
EUR 1200

Anti-ASAH2 Antibody, Rabbit Polyclonal
MBS8110752-01mL 0.1mL
EUR 300

Anti-ASAH2 Antibody, Rabbit Polyclonal
MBS8110752-5x01mL 5x0.1mL
EUR 1200

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot)
IRBAMLASAH2AP270120UL each
EUR 346
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot)
IRBAMLASAH2AP270200UL each
EUR 509
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3)(Western Blot)
MBS8422576-INQUIRE INQUIRE Ask for price

ASAH1/Acid Ceramidase Rabbit anti-Human Polyclonal (N-Terminus) Antibody
MBS2402171-005mL 0.05mL
EUR 615

ASAH1/Acid Ceramidase Rabbit anti-Human Polyclonal (N-Terminus) Antibody
MBS2402171-5x005mL 5x0.05mL
EUR 2605

ASAH2 Rabbit pAb
A12596-100ul 100 ul
EUR 369.6

ASAH2 Rabbit pAb
A12596-200ul 200 ul
EUR 550.8

ASAH2 Rabbit pAb
A12596-20ul 20 ul
EUR 219.6

ASAH2 Rabbit pAb
A12596-50ul 50 ul
EUR 267.6

ASAH2 Rabbit pAb
A7985-100ul 100 ul
EUR 369.6

ASAH2 Rabbit pAb
A7985-200ul 200 ul
EUR 550.8

ASAH2 Rabbit pAb
A7985-20ul 20 ul
EUR 219.6

ASAH2 Rabbit pAb
A7985-50ul 50 ul
EUR 267.6

ASAH2 Rabbit pAb
E2507985 100ul
EUR 225
Description: Available in various conjugation types.

ASAH2 Rabbit pAb
E2511158 100ul
EUR 225
Description: Available in various conjugation types.

ASAH2 Rabbit pAb
E2121910 100ul
EUR 225
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

ASAH2 Rabbit pAb
E2821910 100ul
EUR 225
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

ASAH2 Rabbit pAb
MBS8547174-01mL 0.1mL
EUR 305

ASAH2 Rabbit pAb
MBS8547174-01mLAF405L 0.1mL(AF405L)
EUR 565

ASAH2 Rabbit pAb
MBS8547174-01mLAF405S 0.1mL(AF405S)
EUR 565

ASAH2 Rabbit pAb
MBS8547174-01mLAF610 0.1mL(AF610)
EUR 565

ASAH2 Rabbit pAb
MBS8547174-01mLAF635 0.1mL(AF635)
EUR 565

ASAH2 Rabbit pAb
MBS8548403-01mL 0.1mL
EUR 305

ASAH2 Rabbit pAb
MBS8548403-01mLAF405L 0.1mL(AF405L)
EUR 565

ASAH2 Rabbit pAb
MBS8548403-01mLAF405S 0.1mL(AF405S)
EUR 565

ASAH2 Rabbit pAb
MBS8548403-01mLAF610 0.1mL(AF610)
EUR 565

ASAH2 Rabbit pAb
MBS8548403-01mLAF635 0.1mL(AF635)
EUR 565

ASAH2 Rabbit pAb
A12596 100μL
EUR 1246.96

ASAH2 Rabbit pAb
A7985 20μL
EUR 1246.96

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)
IRBAMLASAH2AP747120UL each
EUR 259
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)
IRBAMLASAH2AP747200UL each
EUR 403
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)
IRBAMLASAH2AP960120UL each
EUR 259
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)

Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)
IRBAMLASAH2AP960200UL each
EUR 403
Description: Rabbit Anti ASAH2 Polyclonal Affinity Purified (PBS with 0.05% sodium azide and 50% glycerol, pH7.4) (Western Blot,IHC,ELISA)

Consequently, a easy change of information treatment-IPD quantification methodology-resulted in a decrease uncertainty of the estimated illicit drug focus, one of many principal steps contributing to the ultimate uncertainty within the normalized each day drug consumption by means of WBE. The present research demonstrated that the employment of IPD may also be very attention-grabbing for future purposes of WBE, particularly when matrix results are excessive, complicating correct quantification. In addition, when a excessive variety of samples and/or compounds have to be analysed, IPD is quicker than calibration and, finally, cost-effective when isotopically labelled inner customary is very costly.