Bottom-up mass spectrometry-based protein evaluation strategies using protease digestion are routinely used to establish and characterize proteins with excessive specificity and sensitivity. Method efficiency is usually measured by sequence protection functionality and the entire variety of attribute peptides recognized, when in comparison with predicted databases. Limitations to generally used solvent-based digestion strategies at present employed embody lengthy digestion occasions (18-24 h or extra), resulting in protease autolysis, which additionally precludes automation, decreases sensitivity, and will increase each intra- and inter-day efficiency variability.
This report describes the event and validation of a easy, 5 min tryptic denaturing natural digestion (DOD) technique for use with tandem mass spectrometry in bottom-up protein identification and characterization. It has been evaluated throughout choose protein toxins and diagnostic scientific protein targets, considerably bettering digestion efficiency when in comparison with different solution-based and enzyme-immobilized strategies. The technique was in comparison with two at present used bottom-up strategies, the 24 h filter-aided pattern prep (FASP) and Flash Digest (1 and Four h) strategies.
Single proteins used to match the strategies included the ricin gentle chain, ricin heavy chain, ricin holotoxin, serotype A Clostridium botulinum toxin, Staphylococcus enterotoxin B, ribonuclease A, and thyroglobulin. In checks, throughout the proteins investigated, the 5 min DOD digestion technique resulted in sequence coverages starting from 55 to 100%, with comparatively excessive reproducibility and precision; outcomes have been higher than or equal to FASP technique outcomes and have been tremendously enhanced when in comparison with Flash technique outcomes.
Importantly, DOD technique intra- and inter-day precision was a lot improved as in comparison with outcomes for each FASP and Flash digestions. These knowledge indicated that the DOD technique, when in comparison with the FASP and Flash Digest strategies, dramatically diminished digestion time, whereas sustaining or bettering the flexibility to detect and characterize focused proteins, and diminished analytical variability for tryptic digestion, leading to markedly quicker and extra exact analyses.
The urinary glucose tetrasaccharide, Glcα1-6Glcα1-4Glcα1-4Glc (Glc4), is a glycogen restrict dextrin that’s elevated in sufferers with glycogen storage illness (GSD) kind III. We evaluated the potential of raw cornstarch remedy to intervene with Glc4 monitoring, by measuring the diurnal variability of Glc4 excretion in sufferers with GSD III. PLHIV reported moderate-severe ache had greater hair cortisone than these reported gentle (p=0.070) or none ache (p=0.014), with no variations between the latter two ache severity teams. Greater ache severity is related to greater hair cortisone ranges amongst Chinese PLHIV.
Voids have been collected at dwelling over 24 hours, saved at 4°C and frozen inside 48 hours. Glc4 was analyzed utilizing liquid chromatography-tandem mass spectrometry and normalized to creatinine. Subjects with GSD III (median age: 13.5 years, vary: 3.7-62; n = 18) accomplished a number of 24-hour urine assortment, and 28/36 collections have been accepted for evaluation. Glc4 was elevated in 16/18 topics (median: 13 mmol/mol creatinine, vary: 2-75, reference vary: <3). In collections with elevated Glc4 (23/28), two-thirds (15/23) had low diurnal variability in Glc4 excretion (coefficient of variation [CV%] <25). The diurnal variability was considerably correlated with the Glc4 focus (Pearson R = .644, P < .05), however not with the dose of raw cornstarch. High intraday variability (>25%) was not persistently noticed in repeat collections by the identical topic.
Greater Pain Severity is Associated with Higher Glucocorticoid Levels in Hair Among a Cohort of People Living with HIV
Pain is a standard prevalence and persistent symptom, which has an antagonistic influence on particular person well-being and high quality of life amongst individuals dwelling with HIV (PLHIV). Alteration within the exercise of the Hypothalamic-Pituitary-Adrenal (HPA) axis leading to irregular glucocorticoid ranges had been proposed to play vital roles in these associations. This research aimed to analyze whether or not ache severity was related to hair glucocorticoid ranges, a novel technique of measuring long-term glucocorticoid publicity, amongst a big cohort of Chinese PLHIV.
A measure of ache severity and hair samples have been collected from 431 adults PLHIV in Guangxi, China. Glucocorticoid (cortisol and cortisone) in hair have been quantified by liquid chromatography-tandem mass spectrometry. The basic linear mannequin was used to check the associations of ache severity with hair glucocorticoid ranges after adjusting for potential confounding elements. Of the 431 PLHIV, 273 reported none ache, 87 reported gentle ache, and 71 reported moderate-severe ache. Hair cortisone, however not hair cortisol, was discovered to vary considerably among the many three ache severity teams (F=3.90, p=0.021). In order to cut back the long-term glucocorticoid ranges, interventions managing ache must be thought of for PLHIV with moderate-severe ache.
Isotope sample deconvolution as a profitable various to calibration curve for software in wastewater-based epidemiology
An isotope sample deconvolution (IPD) quantification technique has been utilized for the willpower of 5 substances (amphetamine, benzoylecgonine, cocaine, methamphetamine and MDMA) in wastewater for the applying in wastewater-based epidemiology (WBE). A beforehand validated technique that used a calibration curve for quantification was modified to use IPD. The two approaches have been in contrast by way of analytical uncertainty in restoration research of high quality management samples, i.e. six wastewater samples from completely different geographical origins spiked at two focus ranges.
Both strategies have been dependable as they handed (z-score < 2) in an interlaboratory train. After 60 particular person determinations, IPD offered 11 outcomes exterior restoration limits (70-120%) whereas the earlier technique produced 31 antagonistic outcomes. All imply values for IPD have been correct whereas 6 out of 10 outcomes confirmed RSD values greater than 30% or recoveries exterior limits when utilizing the previous technique. Moreover, the calculated technique bias for the latter doubles that of IPD, which, in flip, makes the mixed uncertainty (u(c)) a lot greater.
 ASAH2 Polyclonal Antibody |
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| A62106 | EpiGentek | 100 µg | EUR 570.55 |
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Description: fast delivery possible |
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 ASAH2 Rabbit pAb |
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| A12596-100ul | Abclonal | 100 ul | EUR 308 |
ASAH2 Rabbit pAb |
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| A12596-200ul | Abclonal | 200 ul | EUR 459 |
ASAH2 Rabbit pAb |
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| A12596-20ul | Abclonal | 20 ul | EUR 183 |
ASAH2 Rabbit pAb |
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| A12596-50ul | Abclonal | 50 ul | EUR 223 |
ASAH2 Rabbit pAb |
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| A7985-100ul | Abclonal | 100 ul | EUR 308 |
ASAH2 Rabbit pAb |
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| A7985-200ul | Abclonal | 200 ul | EUR 459 |
ASAH2 Rabbit pAb |
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| A7985-20ul | Abclonal | 20 ul | EUR 183 |
ASAH2 Rabbit pAb |
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| A7985-50ul | Abclonal | 50 ul | EUR 223 |
 ASAH2 Antibody |
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| 24731-100ul | SAB | 100ul | EUR 390 |
 ASAH2 antibody |
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| 70R-12124 | Fitzgerald | 100 ug | EUR 403 |
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Description: Rabbit polyclonal ASAH2 antibody |
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ASAH2 Antibody |
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| 36240-100ul | SAB | 100ul | EUR 252 |
ASAH2 Antibody |
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| 3828-100 | Biovision | EUR 316 | |
ASAH2 Antibody |
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| 3828-30T | Biovision | EUR 146 | |
ASAH2 Antibody |
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| 1-CSB-PA002170EA01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
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ASAH2 Antibody |
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| 1-CSB-PA076840 | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
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ASAH2 Antibody |
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| 1-CSB-PA133826 | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
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 ASAH2 Polyclonal Antibody, HRP Conjugated |
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| A62107 | EpiGentek | 100 µg | EUR 570.55 |
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Description: reagents widely cited |
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 ASAH2 Polyclonal Antibody, FITC Conjugated |
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| A62108 | EpiGentek | 100 µg | EUR 570.55 |
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Description: Ask the seller for details |
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 ASAH2 Polyclonal Antibody, Biotin Conjugated |
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| A62109 | EpiGentek | 100 µg | EUR 570.55 |
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Description: The best epigenetics products |
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 ASAH2 Conjugated Antibody |
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| C36240 | SAB | 100ul | EUR 397 |
 Anti-ASAH2 antibody |
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| STJ110292 | St John's Laboratory | 100 µl | EUR 277 |
Anti-ASAH2 antibody |
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| STJ114470 | St John's Laboratory | 100 µl | EUR 277 |
 ASAH2 siRNA |
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| 20-abx900469 | Abbexa |
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ASAH2 siRNA |
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| 20-abx908269 | Abbexa |
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ASAH2 siRNA |
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| 20-abx908270 | Abbexa |
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 ASAH2 Antibody, HRP conjugated |
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| 1-CSB-PA002170EB01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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 ASAH2 Antibody, FITC conjugated |
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| 1-CSB-PA002170EC01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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 ASAH2 Antibody, Biotin conjugated |
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| 1-CSB-PA002170ED01HU | Cusabio |
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Description: A polyclonal antibody against ASAH2. Recognizes ASAH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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 ASAH2 Blocking Peptide |
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| 33R-10941 | Fitzgerald | 50 ug | EUR 191 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ASAH2 antibody, catalog no. 70R-12124 |
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ASAH2 Blocking Peptide |
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| 3828BP-50 | Biovision | EUR 153 | |
 ASAH2 cloning plasmid |
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| CSB-CL865115HU-10ug | Cusabio | 10ug | EUR 474 |
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Description: A cloning plasmid for the ASAH2 gene. |
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 Mouse ASAH2 shRNA Plasmid |
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| 20-abx974501 | Abbexa |
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 Rat ASAH2 shRNA Plasmid |
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| 20-abx987160 | Abbexa |
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 Human ASAH2 shRNA Plasmid |
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| 20-abx961153 | Abbexa |
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) ASAH2 Recombinant Protein (Human) |
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| RP036760 | ABM | 100 ug | Ask for price |
) ASAH2 Recombinant Protein (Mouse) |
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| RP117410 | ABM | 100 ug | Ask for price |
) ASAH2 Recombinant Protein (Rat) |
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| RP191108 | ABM | 100 ug | Ask for price |
 Antibody) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx007107 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| abx025039-400ul | Abbexa | 400 ul | EUR 523 |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| abx025039-80l | Abbexa | 80 µl | EUR 286 |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx214010 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx214011 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx177710 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx173712 | Abbexa |
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N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody |
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| 20-abx303129 | Abbexa |
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 Antibody (HRP)) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (HRP) |
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| 20-abx303130 | Abbexa |
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 Antibody (FITC)) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (FITC) |
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| 20-abx303131 | Abbexa |
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 Antibody (Biotin)) N-Acylsphingosine Amidohydrolase 2 (ASAH2) Antibody (Biotin) |
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| 20-abx303132 | Abbexa |
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 (pORF)) Asah2 ORF Vector (Rat) (pORF) |
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| ORF063704 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) ASAH2 ORF Vector (Human) (pORF) |
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| ORF012254 | ABM | 1.0 ug DNA | EUR 354 |
 (pORF)) Asah2 ORF Vector (Mouse) (pORF) |
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| ORF039138 | ABM | 1.0 ug DNA | EUR 506 |
 (OKCD01831)) ASAH2 ELISA Kit (Human) (OKCD01831) |
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| OKCD01831 | Aviva Systems Biology | 96 Wells | EUR 831 |
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Description: Description of target: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.31 ng/mL |
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) Asah2 sgRNA CRISPR Lentivector set (Rat) |
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| K7087001 | ABM | 3 x 1.0 ug | EUR 339 |
) ASAH2 sgRNA CRISPR Lentivector set (Human) |
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| K0130501 | ABM | 3 x 1.0 ug | EUR 339 |
) Asah2 sgRNA CRISPR Lentivector set (Mouse) |
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| K3499601 | ABM | 3 x 1.0 ug | EUR 339 |
 Protein) Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) Protein |
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| 20-abx654471 | Abbexa |
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 ELISA Kit (CUSTOM)) Mouse ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
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| ELI-34287m | Lifescience Market | 96 Tests | EUR 865 |
 ELISA Kit (CUSTOM)) Human ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
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| ELI-34330h | Lifescience Market | 96 Tests | EUR 824 |
 ELISA Kit (CUSTOM)) Rat ASAH2 (Neutral ceramidase) ELISA Kit (CUSTOM) |
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| ELI-48960r | Lifescience Market | 96 Tests | EUR 886 |
 (Target 1)) Asah2 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7087002 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Asah2 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7087003 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Asah2 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7087004 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) ASAH2 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K0130502 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) ASAH2 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K0130503 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) ASAH2 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K0130504 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 1)) Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3499602 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3499603 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) Asah2 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3499604 | ABM | 1.0 ug DNA | EUR 154 |
 (pPB-C-His)) ASAH2 Protein Vector (Mouse) (pPB-C-His) |
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| PV156550 | ABM | 500 ng | EUR 1065 |
 (pPB-N-His)) ASAH2 Protein Vector (Mouse) (pPB-N-His) |
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| PV156551 | ABM | 500 ng | EUR 1065 |
 (pPM-C-HA)) ASAH2 Protein Vector (Mouse) (pPM-C-HA) |
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| PV156552 | ABM | 500 ng | EUR 1065 |
 (pPM-C-His)) ASAH2 Protein Vector (Mouse) (pPM-C-His) |
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| PV156553 | ABM | 500 ng | EUR 1065 |
 (pPB-C-His)) ASAH2 Protein Vector (Rat) (pPB-C-His) |
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| PV254814 | ABM | 500 ng | EUR 1166 |
 (pPB-N-His)) ASAH2 Protein Vector (Rat) (pPB-N-His) |
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| PV254815 | ABM | 500 ng | EUR 1166 |
 (pPM-C-HA)) ASAH2 Protein Vector (Rat) (pPM-C-HA) |
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| PV254816 | ABM | 500 ng | EUR 1166 |
 (pPM-C-His)) ASAH2 Protein Vector (Rat) (pPM-C-His) |
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| PV254817 | ABM | 500 ng | EUR 1166 |
 (pPB-His-MBP)) ASAH2 Protein Vector (Human) (pPB-His-MBP) |
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| PV324138 | ABM | 500 ng | EUR 481 |
 (pPB-His-GST)) ASAH2 Protein Vector (Human) (pPB-His-GST) |
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| PV324139 | ABM | 500 ng | EUR 481 |
 (pPB-C-His)) ASAH2 Protein Vector (Human) (pPB-C-His) |
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| PV049013 | ABM | 500 ng | EUR 481 |
 (pPB-N-His)) ASAH2 Protein Vector (Human) (pPB-N-His) |
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| PV049014 | ABM | 500 ng | EUR 481 |
 (pPM-C-HA)) ASAH2 Protein Vector (Human) (pPM-C-HA) |
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| PV049015 | ABM | 500 ng | EUR 481 |
 (pPM-C-His)) ASAH2 Protein Vector (Human) (pPM-C-His) |
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| PV049016 | ABM | 500 ng | EUR 329 |
 Asah2 3'UTR GFP Stable Cell Line |
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| TU152183 | ABM | 1.0 ml | Ask for price |
 Asah2 3'UTR Luciferase Stable Cell Line |
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| TU102183 | ABM | 1.0 ml | Ask for price |
Asah2 3'UTR Luciferase Stable Cell Line |
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| TU200951 | ABM | 1.0 ml | Ask for price |
Asah2 3'UTR GFP Stable Cell Line |
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| TU250951 | ABM | 1.0 ml | Ask for price |
 ASAH2 3'UTR GFP Stable Cell Line |
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| TU051220 | ABM | 1.0 ml | EUR 1521 |
 ASAH2 3'UTR Luciferase Stable Cell Line |
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| TU001220 | ABM | 1.0 ml | EUR 1521 |
 ELISA Kit) Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| 20-abx156834 | Abbexa |
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 CLIA Kit) Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) CLIA Kit |
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| 20-abx494694 | Abbexa |
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 (CMV) (pLenti-GIII-CMV)) ASAH2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV656977 | ABM | 1.0 ug DNA | EUR 1355 |
 (UbC) (pLenti-GIII-UbC)) ASAH2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV656981 | ABM | 1.0 ug DNA | EUR 1355 |
 (EF1a) (pLenti-GIII-EF1a)) ASAH2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV656982 | ABM | 1.0 ug DNA | EUR 1355 |
ELISA Kit) Human N-Acylsphingosine Amidohydrolase 2(ASAH2)ELISA Kit |
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| QY-E04735 | Qayee Biotechnology | 96T | EUR 361 |
Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 4731.5 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 477.3 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 639 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| SEE689Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2575.5 |
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Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in serum, plasma, tissue homogenates and other biological fluids. |
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Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) ELISA Kit |
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| 4-SEE689Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human N-Acylsphingosine Amidohydrolase 2 (ASAH2) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
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 GAPDH Rabbit Polyclonal Antibody |
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| 37985-100ul | SAB | 100ul | EUR 252 |
GAPDH Rabbit Polyclonal Antibody |
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| 37985-50ul | SAB | 50ul | EUR 187 |
 EFHD1 Rabbit Polyclonal Antibody |
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| 38001-100ul | SAB | 100ul | EUR 252 |
EFHD1 Rabbit Polyclonal Antibody |
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| 38001-50ul | SAB | 50ul | EUR 187 |
 Alliinase Rabbit Polyclonal Antibody |
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| 38042-100ul | SAB | 100ul | EUR 252 |
Alliinase Rabbit Polyclonal Antibody |
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| 38042-50ul | SAB | 50ul | EUR 187 |
 ECFP Rabbit Polyclonal Antibody |
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| 38077-100ul | SAB | 100ul | EUR 252 |
ECFP Rabbit Polyclonal Antibody |
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| 38077-50ul | SAB | 50ul | EUR 187 |
 EYFP Rabbit Polyclonal Antibody |
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| 38078-100ul | SAB | 100ul | EUR 252 |
EYFP Rabbit Polyclonal Antibody |
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| 38078-50ul | SAB | 50ul | EUR 187 |
 mOrange Rabbit Polyclonal Antibody |
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| 38079-100ul | SAB | 100ul | EUR 252 |
mOrange Rabbit Polyclonal Antibody |
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| 38079-50ul | SAB | 50ul | EUR 187 |
 mStrawberry Rabbit Polyclonal Antibody |
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| 38083-100ul | SAB | 100ul | EUR 252 |
mStrawberry Rabbit Polyclonal Antibody |
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| 38083-50ul | SAB | 50ul | EUR 187 |
 AmCyan Rabbit Polyclonal Antibody |
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| 38086-100ul | SAB | 100ul | EUR 252 |
AmCyan Rabbit Polyclonal Antibody |
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| 38086-50ul | SAB | 50ul | EUR 187 |
 EBFP Rabbit Polyclonal Antibody |
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| 38087-100ul | SAB | 100ul | EUR 252 |
EBFP Rabbit Polyclonal Antibody |
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| 38087-50ul | SAB | 50ul | EUR 187 |
 Vimentin Rabbit Polyclonal Antibody |
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| 38104-100ul | SAB | 100ul | EUR 252 |
Vimentin Rabbit Polyclonal Antibody |
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| 38104-50ul | SAB | 50ul | EUR 187 |
 LDHD Rabbit Polyclonal Antibody |
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| 38105-100ul | SAB | 100ul | EUR 252 |
LDHD Rabbit Polyclonal Antibody |
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| 38105-50ul | SAB | 50ul | EUR 187 |
GAPDH Rabbit Polyclonal Antibody |
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| A01021-005ml | Abbkine | 0.05ml | EUR 147 |
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Description: A polyclonal antibody for detection of GAPDH from Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xneous. This GAPDH antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein |
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GAPDH Rabbit Polyclonal Antibody |
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| A01021-02ml | Abbkine | 0.2ml | EUR 332 |
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Description: A polyclonal antibody for detection of GAPDH from Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xneous. This GAPDH antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein |
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GAPDH Rabbit Polyclonal Antibody |
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| A01021-02ml5 | Abbkine | 0.2ml×5 | EUR 920 |
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Description: A polyclonal antibody for detection of GAPDH from Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xneous. This GAPDH antibody is for WB, IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein |
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 Rabbit Hemoglobin Polyclonal Antibody |
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| A53073 | EpiGentek | 100 µg | EUR 570.55 |
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Description: The best epigenetics products |
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 Met Rabbit Polyclonal Antibody |
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| ABP57458-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of Met from Human, Mouse, Rat. This Met antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of Met of MET |
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Met Rabbit Polyclonal Antibody |
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| ABP57458-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of Met from Human, Mouse, Rat. This Met antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of Met of MET |
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Met Rabbit Polyclonal Antibody |
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| ABP57458-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of Met from Human, Mouse, Rat. This Met antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of Met of MET |
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 VEGF Rabbit Polyclonal Antibody |
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| ABP57460-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of VEGF from Human, Mouse, Rat. This VEGF antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of VEGF of VEGFA |
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VEGF Rabbit Polyclonal Antibody |
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| ABP57460-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of VEGF from Human, Mouse, Rat. This VEGF antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of VEGF of VEGFA |
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VEGF Rabbit Polyclonal Antibody |
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| ABP57460-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of VEGF from Human, Mouse, Rat. This VEGF antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of VEGF of VEGFA |
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 CD10 Rabbit Polyclonal Antibody |
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| ABP57461-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of CD10 from Human, Mouse, Rat. This CD10 antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of CD10 of MME |
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CD10 Rabbit Polyclonal Antibody |
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| ABP57461-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of CD10 from Human, Mouse, Rat. This CD10 antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of CD10 of MME |
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CD10 Rabbit Polyclonal Antibody |
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| ABP57461-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of CD10 from Human, Mouse, Rat. This CD10 antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of CD10 of MME |
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 NM23A Rabbit Polyclonal Antibody |
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| ABP57462-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of NM23A from Human, Mouse, Rat. This NM23A antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of NM23A of NME1 |
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NM23A Rabbit Polyclonal Antibody |
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| ABP57462-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of NM23A from Human, Mouse, Rat. This NM23A antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of NM23A of NME1 |
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NM23A Rabbit Polyclonal Antibody |
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| ABP57462-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of NM23A from Human, Mouse, Rat. This NM23A antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of NM23A of NME1 |
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 ATM Rabbit Polyclonal Antibody |
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| ABP57463-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of ATM from Human, Mouse, Rat. This ATM antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of ATM of ATM |
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ATM Rabbit Polyclonal Antibody |
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| ABP57463-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of ATM from Human, Mouse, Rat. This ATM antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of ATM of ATM |
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ATM Rabbit Polyclonal Antibody |
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| ABP57463-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of ATM from Human, Mouse, Rat. This ATM antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of ATM of ATM |
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ATM Rabbit Polyclonal Antibody |
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| ABP57464-003ml | Abbkine | 0.03ml | EUR 158 |
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Description: A polyclonal antibody for detection of ATM from Human, Mouse, Rat. This ATM antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of ATM of ATM |
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ATM Rabbit Polyclonal Antibody |
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| ABP57464-01ml | Abbkine | 0.1ml | EUR 289 |
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Description: A polyclonal antibody for detection of ATM from Human, Mouse, Rat. This ATM antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of ATM of ATM |
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ATM Rabbit Polyclonal Antibody |
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| ABP57464-02ml | Abbkine | 0.2ml | EUR 414 |
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Description: A polyclonal antibody for detection of ATM from Human, Mouse, Rat. This ATM antibody is for IHC-P. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein of ATM of ATM |
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Consequently, a easy change of information treatment-IPD quantification methodology-resulted in a decrease uncertainty of the estimated illicit drug focus, one of many principal steps contributing to the ultimate uncertainty within the normalized each day drug consumption by means of WBE. The present research demonstrated that the employment of IPD may also be very attention-grabbing for future purposes of WBE, particularly when matrix results are excessive, complicating correct quantification. In addition, when a excessive variety of samples and/or compounds have to be analysed, IPD is quicker than calibration and, finally, cost-effective when isotopically labelled inner customary is very costly.